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	Studies on pathway of biocatalysis producting terephthalic acid by co-metabolism
	Sang P(桑萍) ; Tian J(田晶) ; Gao P(高鹏) ; Xu GW(许国旺)
	2007-06-04
会议名称	dalian international symposia and exhibition on chromatography
会议日期	2007-6-4
会议地点	中国
页码	243/2
通讯作者	许国旺
中文摘要	biological processes are commonly perceived as being less harmful to environment than chemical processes. a variety of chemical routes to synthesize terephthalic acid are known. but biological process to produce terephthalic acid is almost not. the pathway of biocatalysis producting terephthalic acid by co-metabolism of comamonas testosterone strain dsm6577 and stenotrophomonas maltophilia was studied in this thesis. a detecting method of middle metabolites produced at the process of transforming p-xylene to terephthalic acid by hplc was established. the hypersil sax column ( 250 mm ×416 mm ×5μm) was used . the temperature of column was 30°c and detected wavelength at 254 nm. the mobile phase was 0.25 mol/l nh4h2po4 containing 10 % acetonitrile. the flow rate was 0.8 ml/ min. comamonas testosterone strain dsm6577 was shown to be capable of oxidizing p-toluic acid and stenotrophomonas maltophilia was shown to be capable of oxidizing p-xylene as sole souce of carbon into terephthalic acid through sequential reactions catalyzed by a series of enzymes including monooxygenase and dehydrogenase. p-toluic acid, p-xylene’s degradation and erephthalic acid production were studied respectively. the terephthalic acid producing organism comprises the genes encoding enzymes involved in the enzymatic conversion of p-xylene to terephthalic acid as follows: p-xylene was acted by xylene monooygenase to form 4-methylbenzyl alcohol, which was acted by methylbenzyl alcohol dehydrogenase to form p-tolualdehyde. and then, at the action of tolualdehyde dehydrogenase, p-tolualdehyde was transformed to p-toluic acid, which is acted by toluate methyl monooxygenase to form 4-carboxybenzyl alcohol. at last, 4-carboxybenzyl alcohol was acted by carboxybenzyl alcohol methyl dehydrogenase to form 4-carboxybenzaldehyde, which was acted by caboxybenzaldehyde methyl dehydrogenase to form terephthalic acid. this enzymatic pathway is referred to be the biosynthetic pathway of terephthalic acid.
会议主办者	cas等
学科主题	物理化学
语种	中文
ISSN号	978-7-122-00450-5
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/112550]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Sang P,Tian J,Gao P,et al. Studies on pathway of biocatalysis producting terephthalic acid by co-metabolism[C]. 见:dalian international symposia and exhibition on chromatography. 中国. 2007-6-4.

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