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	Strategically searching for estrogenic compounds from Radix puerariae
	Zhang Y(张岩) ; Zhang XZ(张晓哲) ; Zhang FF(章飞芳) ; Xue XY(薛兴亚) ; Xu Q(徐青) ; Liang XM(梁鑫淼)
	2007-06-04
会议名称	dalian international symposia and exhibition on chromatography
会议日期	2007-6-4
会议地点	中国
页码	26/2
通讯作者	梁鑫淼
中文摘要	phytoestrogens have received increasing interest in recent years as possible candidates for alternative hormone replacement therapy (hrt). in this paper, we report one efficient way to search for estrogenic compounds in radix puerariae. preparative hplc was used as a powerful tool for bio-guided separation of the ethyl acetate (etoac) fraction from the crude extract into 29 well-characterized sub-fractions. fractionation was followed by the combination of bioactive screening by recombinant yeast estrogen screening (yes) assay and chemical screening by hplc and/or hplc-ms. ec50 values obtained from yes showed that 10 sub-fractions of f17 to f26 have pronounced estrogenic activities in 103~106 fold less than that response of 17β-estradiol (e2). both biological data and chemical data were correlated. from e2 equivalent (eeq) values, two sub-fractions, f24 and f25, were observed to contribute most to the estrogenic activity of the etoac fraction. four compounds were identified to contribute to the high estrogenic activity in radix puerariae, daidzein, genistein, formononetin and coumestrol, which did not have various isolation and purification steps involved in the traditional approach for bio-guided fractionation. although no novel biological compounds with interesting chemical or spectroscopic features were found, a strategic search method for potential bioactive compounds is presented, which facilitates optimization of an investigation and tedious fractionation of the sample matrices is circumvented.
会议主办者	中国科学院大连化学物理研究所
学科主题	物理化学
语种	中文
内容类型	会议论文
源URL	[http://159.226.238.44/handle/321008/112502]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
推荐引用方式 GB/T 7714	Zhang Y,Zhang XZ,Zhang FF,et al. Strategically searching for estrogenic compounds from Radix puerariae[C]. 见:dalian international symposia and exhibition on chromatography. 中国. 2007-6-4.

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