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	Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag
	Wu, Yudong1,2; Guo, Weisheng3; Peng, Weipan1,2; Zhao, Qan1,2; Piao, Jiafang1,2; Zhang, Bo1,2; Wu, Xiaoli1,2; Wang, Hanjie1,2; Gong, Xiaoqun1,2; Chang, Jin1,2
刊名	Acs applied materials & interfaces
	2017-03-22
卷号	9期号:11页码:9369-9377
关键词	Fluorescence enzyme-linked immunosorbent assay Gold nanoparticles Human alpha-thrombin Alpha fetoprotein Hepatitis b virus surface antigen
ISSN号	1944-8244
DOI	10.1021/acsami.6b16236
通讯作者	Gong, xiaoqun(gongxiaoqun@tju.edu.cn) ; Chang, jin(jinchang@tju.edu.cn)
英文摘要	At present, enzyme-linked immunosorbent assay (elisa) is considered to be the most appropriate approach in clinical biomarker detection, with good specificity, low cost, arid straightforward readout. however, unsatisfactory sensitivity severely hampers its wide application in clinical diagnosis. herein, we designed a new kind of enhanced fluorescence enzyme-inked immunosorbent assay (felisa) based on the human alpha-thrombin (hat) triggering fluorescence "turn-on" signals. in this system, detection antibodies (ab(2)) and hat were labeled on the gold nanoparticles (aunps) to form the detection probes, and a bisamide derivative of rhodamineno(110) with fluorescence quenched served as the substrate of hat. after the sandwich immunoreaction, hat on the sandwich structure could catalyze the cleavage of the fluorescence-quenched substrate, leading to a strong fluorescence signal for sensing ultralow levels of alpha fetoprotein (afp) and hepatitis b virus surface antigen (hbsag). under the optimized reaction conditions, afp and hbsag were detected at the ultralow concentrations of 10(-8) ng ml(-1) and 5 x 10(-4) iu ml(-i), respectively, which were at least 10(4) times lower than those of the conventional fluorescence assay and 106 times lower than those of the conventional elisa. in addition, we further discussed the efficiency of the sensitive felisa in clinical serum samples, showing great potential in practical applications.
WOS关键词	LINKED-IMMUNOSORBENT-ASSAY ; PROSTATE-SPECIFIC ANTIGEN ; GOLD NANOPARTICLES ; SENSITIVE DETECTION ; ALPHA-FETOPROTEIN ; CHEMILUMINESCENCE IMMUNOASSAY ; IMMUNOCHROMATOGRAPHIC ASSAY ; SIGNAL AMPLIFICATION ; SILVER NANOPARTICLES ; PLASMONIC ELISA
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000397478100020
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176683
专题	高能物理研究所
通讯作者	Gong, Xiaoqun; Chang, Jin
作者单位	1.Tianjin Univ, Sch Life Sci, Sch Mat Sci & Engn, 92 Weijin Rd, Tianjin 300072, Peoples R China 2.Tianjin Engn Ctr Micro Nano Biomat & Detect Treat, 92 Weijin Rd, Tianjin 300072, Peoples R China 3.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Wu, Yudong,Guo, Weisheng,Peng, Weipan,et al. Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag[J]. Acs applied materials & interfaces,2017,9(11):9369-9377.
APA	Wu, Yudong.,Guo, Weisheng.,Peng, Weipan.,Zhao, Qan.,Piao, Jiafang.,...&Chang, Jin.(2017).Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag.Acs applied materials & interfaces,9(11),9369-9377.
MLA	Wu, Yudong,et al."Enhanced fluorescence elisa based on hat triggering fluorescence "turn-on" with enzyme-antibody dual labeled aunp probes for ultrasensitive detection of afp and hbsag".Acs applied materials & interfaces 9.11(2017):9369-9377.

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