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	Nano-cage-mediated refolding of insulin by peg-pe micelle
	Fang, Xiaocui1,2; Yang, Tao1,3; Wang, Luoyang1,3; Yu, Jibing1; Wei, Xiuli1; Zhou, Yinjian1; Wang, Chen2; Liang, Wei1
刊名	Biomaterials
	2016
卷号	77页码:139-148
关键词	Insulin Dtt Refolding Peg-pe micelle Nano-cage Negative charge layer
ISSN号	0142-9612
DOI	10.1016/j.biomaterials.2015.11.007
通讯作者	Wang, chen(wangch@nanoctr.cn)
英文摘要	Insulin aggregation has pronounced pharmaceutical implications and biological importance. deposition of insulin aggregates is associated with type ii diabetes and instability of pharmaceutical formulations. we present in this study the renaturation effect of peg-pe micelle on dithiothreitol (dtt)-denatured insulin revealed by techniques including turbidity assay, circular dichroism (cd), thioflavint (tht) binding assay, bis-ans binding assay, agarose gel electrophoresis and maldi-tof ms. the obtained results show that peg-pe micelle having a hydrophilic nano-cage-like structure in which with a negative charge layer, can capture dtt-induced insulin a and b chains, and block their hydrophobic interaction, thereby preventing aggregation. the reduced insulin a and b chain in the nano-cage are capable of recognizing each other and form the native insulin with yields of similar to 30% as measured by hypoglycemic activity analysis in mice. the observed insulin refolding assisted by peg-pe micelle may be applicable to other proteins. (c) 2015 published by elsevier ltd.
WOS关键词	PROTEIN DISULFIDE-ISOMERASE ; CARBONIC ANHYDRASE-B ; AMYLOID FIBRILS ; IN-VIVO ; PHOSPHOLIPID MICELLES ; MOLECULAR CHAPERONES ; POLYMERIC MICELLES ; LIQUID INTERFACES ; DRUG-DELIVERY ; AGGREGATION
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000367118200013
内容类型	期刊论文
URI标识	http://www.corc.org.cn/handle/1471x/2176433
专题	高能物理研究所
通讯作者	Wang, Chen
作者单位	1.Chinese Acad Sci, Inst Biophys, Prot & Peptide Pharmaceut Lab, Beijing 100101, Peoples R China 2.Natl Ctr Nanosci & Technol, CAS Key Lab Biol Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fang, Xiaocui,Yang, Tao,Wang, Luoyang,et al. Nano-cage-mediated refolding of insulin by peg-pe micelle[J]. Biomaterials,2016,77:139-148.
APA	Fang, Xiaocui.,Yang, Tao.,Wang, Luoyang.,Yu, Jibing.,Wei, Xiuli.,...&Liang, Wei.(2016).Nano-cage-mediated refolding of insulin by peg-pe micelle.Biomaterials,77,139-148.
MLA	Fang, Xiaocui,et al."Nano-cage-mediated refolding of insulin by peg-pe micelle".Biomaterials 77(2016):139-148.

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