A novel serine protease with clip domain from scallop Chlamys farreri

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	A novel serine protease with clip domain from scallop Chlamys farreri
	Zhu, L ; Song, LS ; Mao, Y ; Zhao, JM ; Li, CH ; Xu, W
刊名	MOLECULAR BIOLOGY REPORTS
	2008
卷号	35 期号:2 页码:257-264
关键词	Chlamys farreri clip domain immune response mRNA expression serine protease tissue distribution
ISSN号	0301-4851
通讯作者	llingzhu@163.com ; lshsong@ms.qdio.ac.cn
中文摘要	The serine proteases with clip domain are involved in various innate immune functions in invertebrate such as antimicrobial activity, cell adhesion, pattern recognition and regulation of the prophenoloxidase system. A serine protease with clip-domain cDNA (Cf SP) was obtained by Expressed sequence taggings (ESTs) method and rapid amplification of cDNA ends (RACE). The Cf SP full-length cDNA was of 1,152 bp, including a 5'-terminal untranslated region (UTR) of 63 bp, a 3'-terminal UTR of 81 bp with a canonical polyadenylation signal sequence AATAAA and a poly(A) tail, and an open reading frame of 1,008 bp encoding a polypeptide of 336 amino acids with a putative signal peptide of 19 amino acids. The deduced amino acid sequence of Cf SP contained an amino-terminal clip domain with three disulfide bonds formed six conserved Cys residues, a carboxyl-terminal trypsin-like domain with the conserved His-Asp-Ser catalytic triad, and a low complexity linker sequence. The Cf SP was strongly expressed in hemocytes and the mRNA expression of Cf SP was up-regulated and increased 3.2-fold and 2.6-fold at 16 h after injection of Vibrio anguillarum and Micrococcus luteus. The results suggested that Cf SP gene might be involved in immune response of Gram-negative and Gram-positive microbial infection in scallop.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
原文出处	JUN
WOS记录号	WOS:000256082300024
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/6013]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Zhu, L,Song, LS,Mao, Y,et al. A novel serine protease with clip domain from scallop Chlamys farreri[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):257-264.
APA	Zhu, L,Song, LS,Mao, Y,Zhao, JM,Li, CH,&Xu, W.(2008).A novel serine protease with clip domain from scallop Chlamys farreri.MOLECULAR BIOLOGY REPORTS,35(2),257-264.
MLA	Zhu, L,et al."A novel serine protease with clip domain from scallop Chlamys farreri".MOLECULAR BIOLOGY REPORTS 35.2(2008):257-264.