Alkaline phosphatase activity of phytoplankton in East China Sea coastal waters with frequent harmful algal bloom occurrences

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Alkaline phosphatase activity of phytoplankton in East China Sea coastal waters with frequent harmful algal bloom occurrences
	Huang, BQ ; Ou, LJ ; Wang, XL ; Huo, WY ; Li, RX ; Hong, HS ; Zhu, MY ; Qi, YZ
刊名	AQUATIC MICROBIAL ECOLOGY
	2007
卷号	49 期号:2 页码:195-206
关键词	alkaline phosphatase phosphorus stress phytoplankton harmful algal blooms East China Sea
ISSN号	0948-3055
通讯作者	hshong@xmu.edu.cn
中文摘要	Alkaline phosphatase activity (APA) was measured during 3 cruises in spring 2002, 2003 and 2005 using bulk and single-cell assays in coastal waters in the East China Sea which experience frequent harmful algal bloom occurrences. The bulk APA ranged from below the detection limit to 73.53 nmol l(-1) h(-1), with mean values of 15.73 +/- 14.12, 23.77 +/- 15.20 and 11.48 +/- 12.44 nmol l(-1) h(-1) for 2002, 2003 and 2005, respectively. The cell-bound fraction (mainly phytoplankton) was the major contributor to APA, with averages of 74 and 80% during 2003 and 2005, respectively. During the spring of 2005, most of the dominant dinoflagellates had high percentages of enzyme-labeled fluorescence (ELF)-labeled cells, while only a few diatoms and chrysophytes were labeled with ELF. Among the dinoflagellates, Protoperidinium spp. and Karenia mikimotoi had the highest percentages of ELF-labeled cells (84 and 82 %, respectively), whereas Gonyaulax spp. and Dinophysis spp. had the lowest percentages of labeled species (17 and 21 %, respectively). An alkaline phosphatase (AP) kinetic experiment was performed during 2005, with a turnover time of 10 h and a maximum potential velocity of 206.1 nmol l(-1) h(-1). The present results showed that severe phosphorus (P) stress occurred during springs in the study area, in particular when Prorocentrum donghaiense bloomed during 2003. AP played an important role in hydrolyzing soluble nonreactive phosphorus. The dominant dinoflagellates, which suffered more severe P stress compared to the diatoms, were the major AP producers during the spring of 2005, and differences in physiological P status existed among different dinoflagellate species.
学科主题	Ecology; Marine & Freshwater Biology; Microbiology
收录类别	SCI
原文出处	INTER-RESEARCH
WOS记录号	WOS:000251749300008
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5839]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Huang, BQ,Ou, LJ,Wang, XL,et al. Alkaline phosphatase activity of phytoplankton in East China Sea coastal waters with frequent harmful algal bloom occurrences[J]. AQUATIC MICROBIAL ECOLOGY,2007,49(2):195-206.
APA	Huang, BQ.,Ou, LJ.,Wang, XL.,Huo, WY.,Li, RX.,...&Qi, YZ.(2007).Alkaline phosphatase activity of phytoplankton in East China Sea coastal waters with frequent harmful algal bloom occurrences.AQUATIC MICROBIAL ECOLOGY,49(2),195-206.
MLA	Huang, BQ,et al."Alkaline phosphatase activity of phytoplankton in East China Sea coastal waters with frequent harmful algal bloom occurrences".AQUATIC MICROBIAL ECOLOGY 49.2(2007):195-206.