Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 热带海洋环境国家重点实验室(LTO)

	Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis
	Shao, P ; Chen, YQ ; Zhou, H ; Yuan, J ; Qu, LH ; Zhao, D ; Lin, YS
刊名	MARINE BIOLOGY
	2004
卷号	144 期号:2 页码:215-224
ISSN号	0025-3162
通讯作者	lsbrc04@zsu.edu.cn
中文摘要	The internal transcribed spacer (ITS) regions and 5.8S rRNA genes from several strains of toxic Gymnodiniaceae were sequenced and subjected to phylogenetic analysis with other Gymnodiniaceae species. Sequence comparisons showed that high sequence divergence existed in Gymnodiniaceae, especially in the genus Gymnodinium. The amplicons of the ITS regions from Amphidinium and Gyrodinium species were 438-439 and 604-605 bp, respectively, and those of the Gymnodinium species ranged from 575 to 615 bp. The mean distance value within Gymnodinium, calculated from the ITS sequence, was 0.68827 (range: 0-0.92323), 0.11342 for Amphidinium (range: 0.00467-0.17120) and 0.2005 for Karenia (range: 0.00521-0.29971). Low distance values were found within the species Gyrodinium instriatum (<0.01) and Karlodinium micrum (<0.02). Amphidinium remarkably had a shorter ITS than did other genera in Gymnodiniaceae, this implied that Amphidinium might be distant from the other Gymnodiniaceae and supported Saunders' opinion that the taxonomy of Amphidinium needs to be reevaluated. Largely congruent phylogenetic trees were produced by the maximum-parsimony method (PAUP), maximum-likelihood method (PAUP) and Bayesian inference (MrBayes), whereas the three analyses showed that the genera Gymnodinium and Karenia are unresolved groups in phylogeny. Minor sequence divergence was found within the different clones of Amphidinium carterae, suggesting that the ITS regions are suitable as genus- and species-specific oligonucleotide probes to rapidly detect and identify the red tide-forming algal species.
学科主题	Marine & Freshwater Biology
收录类别	SCI
原文出处	SPRINGER-VERLAG
WOS记录号	WOS:000188496200002
公开日期	2011-07-03
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/5671]
专题	南海海洋研究所_热带海洋环境国家重点实验室(LTO)
推荐引用方式 GB/T 7714	Shao, P,Chen, YQ,Zhou, H,et al. Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis[J]. MARINE BIOLOGY,2004,144(2):215-224.
APA	Shao, P.,Chen, YQ.,Zhou, H.,Yuan, J.,Qu, LH.,...&Lin, YS.(2004).Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis.MARINE BIOLOGY,144(2),215-224.
MLA	Shao, P,et al."Genetic variability in Gymnodiniaceae ITS regions: implications for species identification and phylogenetic analysis".MARINE BIOLOGY 144.2(2004):215-224.