Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection

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	Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection
	Zhou, Xiuxia 1,2; Wang, Lu 1,2; Feng, Hong 1,2; Guo, Qionglin 1; Dai, Heping 1
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2011-04-01
卷号	35 期号:4 页码:441-451
关键词	Innate immunity Acute phase response (APR) Acute phase protein (APP) Serum amyloid A (SAA) Bacterial infection Chinese soft-shelled turtle (Trionyx sinensis)
ISSN号	0145-305X
通讯作者	Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China ; qlguo@ihb.ac.cn
中文摘要	Chinese soft-shelled turtle (Trionyx sinensis) is an important culture reptile. However, little is known about its acute phase response (APR) caused by bacteria. Serum amyloid A (SAA) is a major acute phase protein (APP). In this study, a turtle SAA homologue was identified and described in reptiles. The full-length cDNA of turtle SAA was 554 bp and contained a 381 bp open reading frame (ORF) coding for a protein of 127 aa. Similar to other known SAA genes, the turtle SAA gene contained three exons and two introns. The promoter region of turtle SAA gene contained the consensus binding sites for nuclear factor (NF)-kappa B and c-Rel. The turtle SAA amino acid sequence shared the highest identity to avian SAA sequences. Meantime, we present the first systematic study with expression levels of five genes encoding APPs in immune response caused by Aeromonas hydrophila infection. After infection, turtle SAA mRNA was induced in liver at 8 h, then increased more than 1200-fold at 2 d: in spleen and kidney, the SAA mRNAs were also induced during 8 h-7 d, but the level was far lower than that in the liver. The complement 3 (C3), fibrinogen-gamma chain (Fb-G) and cathepsin L (CathL) mRNAs were increased in liver at 2d, whereas the albumin (ALB) mRNA was significantly decreased during 8 h-7 d. Our studies suggest that the APR in turtle with A. hydrophila infection is similar to that in mammals, and SAA is a major indicator of bacterial infection, especially at early stage, in reptiles. Additionally, the different expression patterns of five APP genes observed in present studies could provide clues for understanding the innate immune mechanisms in the APR of reptiles. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
英文摘要	Chinese soft-shelled turtle (Trionyx sinensis) is an important culture reptile. However, little is known about its acute phase response (APR) caused by bacteria. Serum amyloid A (SAA) is a major acute phase protein (APP). In this study, a turtle SAA homologue was identified and described in reptiles. The full-length cDNA of turtle SAA was 554 bp and contained a 381 bp open reading frame (ORF) coding for a protein of 127 aa. Similar to other known SAA genes, the turtle SAA gene contained three exons and two introns. The promoter region of turtle SAA gene contained the consensus binding sites for nuclear factor (NF)-kappa B and c-Rel. The turtle SAA amino acid sequence shared the highest identity to avian SAA sequences. Meantime, we present the first systematic study with expression levels of five genes encoding APPs in immune response caused by Aeromonas hydrophila infection. After infection, turtle SAA mRNA was induced in liver at 8 h, then increased more than 1200-fold at 2 d: in spleen and kidney, the SAA mRNAs were also induced during 8 h-7 d, but the level was far lower than that in the liver. The complement 3 (C3), fibrinogen-gamma chain (Fb-G) and cathepsin L (CathL) mRNAs were increased in liver at 2d, whereas the albumin (ALB) mRNA was significantly decreased during 8 h-7 d. Our studies suggest that the APR in turtle with A. hydrophila infection is similar to that in mammals, and SAA is a major indicator of bacterial infection, especially at early stage, in reptiles. Additionally, the different expression patterns of five APP genes observed in present studies could provide clues for understanding the innate immune mechanisms in the APR of reptiles. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.
学科主题	Immunology; Zoology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Immunology ; Zoology
研究领域[WOS]	Immunology ; Zoology
关键词[WOS]	SERUM-AMYLOID-A ; CARP CYPRINUS-CARPIO ; GENE-EXPRESSION ; PROTEINS ; TRANSCRIPTION ; INFLAMMATION ; FIBRINOGEN ; LIPOPOLYSACCHARIDE ; BACTERIAL ; CELLS
收录类别	SCI
资助信息	National Natural Science Foundation of China [30070588, 30871912]; National Basic Research Program of China [2009CB118704]
语种	英语
WOS记录号	WOS:000288819800005
公开日期	2011-06-30
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15626]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Xiuxia,Wang, Lu,Feng, Hong,et al. Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2011,35(4):441-451.
APA	Zhou, Xiuxia,Wang, Lu,Feng, Hong,Guo, Qionglin,&Dai, Heping.(2011).Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,35(4),441-451.
MLA	Zhou, Xiuxia,et al."Acute phase response in Chinese soft-shelled turtle (Trionyx sinensis) with Aeromonas hydrophila infection".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 35.4(2011):441-451.