Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)
	Guan, Bo 1,2; Ma, Hong 3; Wang, Yaping 1; Hu, Yuanlei 3; Lin, Zhongping 3; Zhu, Zuoyan 1; Hu, Wei 1
刊名	MARINE BIOTECHNOLOGY
	2011-04-01
卷号	13 期号:2 页码:336-344
关键词	Vitreoscilla hemoglobin (VHb) Hypoxia Zebrafish (Danio rerio) Transgenic fish
ISSN号	1436-2228
通讯作者	Hu,W. (huwei@ihb.ac.cn) ; Hu, Y.(huyl@pku.edu.cn)
中文摘要	Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F2 transgenic fish was significantly greater than that of the sibling controls under 2.5% O2 (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish. © 2010 Springer Science+Business Media, LLC.
英文摘要	Aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. Here, we used the vector pCVCG expressing the Vitreoscilla hemoglobin (vhb) gene driven by the common carp beta-actin promoter to create stable transgenic zebrafish. The survival rate of the 7-day-old F(2) transgenic fish was significantly greater than that of the sibling controls under 2.5% O(2) (dissolved oxygen (DO), 0.91 mg/l). Meanwhile, we investigated the relative expression levels of several marker genes (hypoxia-inducible factor alpha 1, heat shock cognate 70-kDa protein, erythropoietin, beta and alpha globin genes, lactate dehydrogenase, catalase, superoxide dismutase, and glutathione peroxidase) of transgenic fish and siblings after hypoxia exposure for 156 h. The expression profiles of the vhb transgenic zebrafish revealed that VHb could partially alleviate the hypoxia stress response to improve the survival rate of the fish. These results suggest that that vhb gene may be an efficient candidate for genetically modifying hypoxia tolerance in fish.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
关键词[WOS]	RELIEVES NITROSATIVE STRESS ; BACTERIAL HEMOGLOBIN ; ESCHERICHIA-COLI ; RAINBOW-TROUT ; 2,4-DINITROTOLUENE DEGRADATION ; AQUATIC HYPOXIA ; BURKHOLDERIA SP ; EXPRESSION ; GENE ; FISH
收录类别	SCI
语种	英语
WOS记录号	WOS:000290039300024
公开日期	2011-03-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/14460]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Guan, Bo,Ma, Hong,Wang, Yaping,et al. Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)[J]. MARINE BIOTECHNOLOGY,2011,13(2):336-344.
APA	Guan, Bo.,Ma, Hong.,Wang, Yaping.,Hu, Yuanlei.,Lin, Zhongping.,...&Hu, Wei.(2011).Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio).MARINE BIOTECHNOLOGY,13(2),336-344.
MLA	Guan, Bo,et al."Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in Zebrafish (Danio rerio)".MARINE BIOTECHNOLOGY 13.2(2011):336-344.