Fish germ cells

CORC > 水生生物研究所 > 鱼类生物学及渔业生物技术研究中心 > 期刊论文

	Fish germ cells
	Xu HongYan 2; Li MingYou 2; Gui JianFang 1; Hong YunHan 1,2
刊名	SCIENCE CHINA-LIFE SCIENCES
	2010-04-01
卷号	53 期号:4 页码:435-446
关键词	fish germ cell germ plasm reproduction reproductive technology semi-cloning
ISSN号	1674-7305
通讯作者	Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Fish, like many other animals, have two major cell lineages, namely the germline and soma. The germ-soma separation is one of the earliest events of embryonic development. Germ cells can be specifically labeled and isolated for culture and transplantation, providing tools for reproduction of endangered species in close relatives, such as surrogate production of trout in salmon. Haploid cell cultures, such as medaka haploid embryonic stem cells have recently been obtained, which are capable of mimicking sperm to produce fertile offspring, upon nuclear being directly transferred into normal eggs. Such fish originated from a mosaic oocyte that had a haploid meiotic nucleus and a transplanted haploid mitotic cell culture nucleus. The first semi-cloned fish is Holly. Here we review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology.
英文摘要	Fish, like many other animals, have two major cell lineages, namely the germline and soma. The germ-soma separation is one of the earliest events of embryonic development. Germ cells can be specifically labeled and isolated for culture and transplantation, providing tools for reproduction of endangered species in close relatives, such as surrogate production of trout in salmon. Haploid cell cultures, such as medaka haploid embryonic stem cells have recently been obtained, which are capable of mimicking sperm to produce fertile offspring, upon nuclear being directly transferred into normal eggs. Such fish originated from a mosaic oocyte that had a haploid meiotic nucleus and a transplanted haploid mitotic cell culture nucleus. The first semi-cloned fish is Holly. Here we review the current status and future directions of understanding and manipulating fish germ cells in basic research and reproductive technology.
学科主题	Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biology
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
关键词[WOS]	MEDAKA ORYZIAS-LATIPES ; VASA-LIKE GENE ; TROUT ONCORHYNCHUS-MYKISS ; TRANSGENIC RAINBOW-TROUT ; EMBRYONIC STEM-CELLS ; CHEMOKINE RECEPTOR ; IN-VITRO ; FLUORESCENT PROTEIN ; LINE CHIMERAS ; ZEBRAFISH
收录类别	SCI
资助信息	Biomedical Research Council of Singapore [R-05-1-21-19-404, R-08-1-21-19-585, SBIC-SSCC C-002-2007]; Ministry of Education of Singapore [R-154-000-285-112]; National University of Singapore [R-154-000-153-720]; National Key Basic Research Program of China [2004CB117406, 2010CB126301]
语种	英语
WOS记录号	WOS:000277417800006
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13625]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Natl Univ Singapore, Dept Biol Sci, Singapore 119260, Singapore
推荐引用方式 GB/T 7714	Xu HongYan,Li MingYou,Gui JianFang,et al. Fish germ cells[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(4):435-446.
APA	Xu HongYan,Li MingYou,Gui JianFang,&Hong YunHan.(2010).Fish germ cells.SCIENCE CHINA-LIFE SCIENCES,53(4),435-446.
MLA	Xu HongYan,et al."Fish germ cells".SCIENCE CHINA-LIFE SCIENCES 53.4(2010):435-446.