Fish virus-induced interferon exerts antiviral function through Stat1 pathway

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	Fish virus-induced interferon exerts antiviral function through Stat1 pathway
	Yu, Fei-Fei; Zhang, Yi-Bing; Liu, Ting-Kai; Liu, Ying; Sun, Fan; Jiang, Jun; Gui, Jian-Fang
刊名	MOLECULAR IMMUNOLOGY
	2010-08-01
卷号	47 期号:14 页码:2330-2341
关键词	Fish virus induced Interferon stat1 Interferon stimulated genes (ISGs) Antiviral response Jak Stat signaling pathway Positive feedback loop Recombinant protein Transfection
ISSN号	0161-5890
通讯作者	Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
中文摘要	Virus-Induced interferons (IFNs) have been identified in various fish species and display antiviral activities similar to mammalian type I IFNs However apart from the mammalian IFN system the IFN signaling pathway remains largely unknown Using transient transfection and recombinant protein we are reporting in this study that a crucian carp (Carassius auratus L) IFN exhibits strong antiviral activity against grass carp hemorrhagic virus (GCHV) infection and also mediates Poly I C-Induced antiviral response which correlates with its ability to Induce a set of IFN-stimulated genes (ISGs) Strikingly overexpression of wild-type Stat1 increases the effects of IFN on both the expression of ISGs and the inhibition of virus infection whereas a dominant negative mutant of Stat1 (Stat1-Delta C) which lacks of the C-terminal transcriptional activation domain (TAD) inhibits the antiviral activity of IFN and reduces the expression of ISGs demonstrating that fish IFN induces the expression of ISGs and host antiviral response through Stat1 pathway reminiscent that of mammalian IFNs Significantly unlike mammalian type I IFNs recombinant fish IFN is able to upregulate IFN itself which is enhanced by overexpression of Stat1 but impaired by knockdown of Stat1 indicating a positive feedback loop in regulation of IFN itself These results provide strong evidence for the existence of an evolutionary conserved Stat1 pathway between fish and mammals which is indispensable for fish virus-induced IFN antiviral response (C) 2010 Elsevier Ltd All rights reserved
英文摘要	Virus-Induced interferons (IFNs) have been identified in various fish species and display antiviral activities similar to mammalian type I IFNs However apart from the mammalian IFN system the IFN signaling pathway remains largely unknown Using transient transfection and recombinant protein we are reporting in this study that a crucian carp (Carassius auratus L) IFN exhibits strong antiviral activity against grass carp hemorrhagic virus (GCHV) infection and also mediates Poly I C-Induced antiviral response which correlates with its ability to Induce a set of IFN-stimulated genes (ISGs) Strikingly overexpression of wild-type Stat1 increases the effects of IFN on both the expression of ISGs and the inhibition of virus infection whereas a dominant negative mutant of Stat1 (Stat1-Delta C) which lacks of the C-terminal transcriptional activation domain (TAD) inhibits the antiviral activity of IFN and reduces the expression of ISGs demonstrating that fish IFN induces the expression of ISGs and host antiviral response through Stat1 pathway reminiscent that of mammalian IFNs Significantly unlike mammalian type I IFNs recombinant fish IFN is able to upregulate IFN itself which is enhanced by overexpression of Stat1 but impaired by knockdown of Stat1 indicating a positive feedback loop in regulation of IFN itself These results provide strong evidence for the existence of an evolutionary conserved Stat1 pathway between fish and mammals which is indispensable for fish virus-induced IFN antiviral response (C) 2010 Elsevier Ltd All rights reserved
学科主题	Biochemistry & Molecular Biology; Immunology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Immunology
研究领域[WOS]	Biochemistry & Molecular Biology ; Immunology
关键词[WOS]	REGULATORY FACTOR FAMILY ; INNATE IMMUNE-RESPONSE ; ATLANTIC SALMON ; CELL-LINE ; PARALICHTHYS-OLIVACEUS ; TRANSCRIPTION FACTORS ; TARGETED DISRUPTION ; SIGNAL-TRANSDUCTION ; NECROSIS VIRUS ; DANIO-RERIO
收录类别	SCI
资助信息	973 National Basic Research Program of China [2010CB126303]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30671617 30871922]; National transgenic project [2009ZX08010-021B]
语种	英语
WOS记录号	WOS:000284304700005
公开日期	2010-12-23
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/13569]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Yu, Fei-Fei,Zhang, Yi-Bing,Liu, Ting-Kai,et al. Fish virus-induced interferon exerts antiviral function through Stat1 pathway[J]. MOLECULAR IMMUNOLOGY,2010,47(14):2330-2341.
APA	Yu, Fei-Fei.,Zhang, Yi-Bing.,Liu, Ting-Kai.,Liu, Ying.,Sun, Fan.,...&Gui, Jian-Fang.(2010).Fish virus-induced interferon exerts antiviral function through Stat1 pathway.MOLECULAR IMMUNOLOGY,47(14),2330-2341.
MLA	Yu, Fei-Fei,et al."Fish virus-induced interferon exerts antiviral function through Stat1 pathway".MOLECULAR IMMUNOLOGY 47.14(2010):2330-2341.