Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis

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	Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis
	Hou, Yansong ; Liang, Wei ; Zhang, Liping ; Cheng, Shuiping ; He, Feng ; Wu, Zhenbin
刊名	FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA
	2011
卷号	5 期号:1 页码:84-91
关键词	high-performance liquid chromatographic (HPLC) algae pigment chemotaxonomy
通讯作者	Liang, W, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China ; liangwei02@tsinghua.org.cn, wuzb@ihb.ac.cn
中文摘要	The study of community composition of algae is essential for understanding the structure and dynamics of the aquatic ecosystem and for evaluating the eutrophic level of the water body. A high-performance liquid chromatographic (HPLC) method based on a reversephase C-18 nonpolar column was developed for the main algal taxa, which includes cyanophytes, bacillariophytes, euglenophytes, dinophytes, and chlorophytes. Based on the elution order using HPLC, 19 pigments were identified, and they were chlorophyllide a, 19'-butanoyloxyfucoxanthin, chlorophyll c (1) + c (2), phephorbides a, peridinin, methyl-chlorophyllide a, fucoxanthin, neoxanthin, violaxanthin, myxoxanthophyll, diadinoxanthin, diatoxanthin, lutein, zeaxanthin, chlorophyll b allomer, chlorophyll b, chlorophyll a allomer, chlorophyll a, and beta,beta-carotene. A comparison study of cell microscopic counts and accessory pigment analysis indicated that HPLC analysis could be a useful tool for monitoring phytoplankton communities and their abundance.
学科主题	Engineering ; Environmental; Environmental Sciences
收录类别	SCI
资助信息	National Key Science Research Project [2009ZX07106-003, 2008ZX07316-004]; CAS [TJZX2-YW-07]; Jiangsu Province project [BE2008651]; Agricultural Science and Technology Achievement Transformation Project [2009GB2A100015]
公开日期	2011-04-14
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/15508]
专题	水生生物研究所_水环境工程研究中心_期刊论文
推荐引用方式 GB/T 7714	Hou, Yansong,Liang, Wei,Zhang, Liping,et al. Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis[J]. FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA,2011,5(1):84-91.
APA	Hou, Yansong,Liang, Wei,Zhang, Liping,Cheng, Shuiping,He, Feng,&Wu, Zhenbin.(2011).Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis.FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA,5(1),84-91.
MLA	Hou, Yansong,et al."Freshwater algae chemotaxonomy by high-performance liquid chromatographic (HPLC) analysis".FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA 5.1(2011):84-91.