Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS

	Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS
	Kong, Liang ; Yu, Zhiyuan ; Bao, Yongming ; Su, Xingye ; Zou, Hanfa ; Li, Xin
刊名	analytical and bioanalytical chemistry
	2006-09-01
卷号	386 期号:2 页码:264-274
关键词	coniferyl ferulate ferulic acid ethyl ester HPLC-MS rhizoma chuanxiong traditional Chinese medicine
ISSN号	1618-2642
通讯作者	邹汉法
产权排序	1;1
英文摘要	a new screening and analysis method that combines in vitro metabolism with high-performance liquid chromatography-mass spectrometry (hplc-ms) was developed for the screening and analysis of an antineoplastic compound, coniferyl ferulate, which is present in the rhizome of rhizoma chuanxiong. infrared (ir), ultraviolet visible spectroscopy (uv-vis), nuclear magnetic resonance (nmr) and element analysis were used to identify the molecular structure of coniferyl ferulate. the quantitative analysis of coniferyl ferulate in different extracts of rhizoma chuanxiong was carried out, and the metabolism of coniferyl ferulate was investigated by in vitro incubation with rat liver homogenate. the metabolite of coniferyl ferulate, ferulic acid ethyl ester, was identified by hplc-ms, uv-vis and ir. in addition, antineoplastic activities of coniferyl ferulate and ferulic acid ethyl ester were detected by the mtt assay. the observed inhibition rate of coniferyl ferulate on the activity of hela cells was over 80% at 5.4 ng mu l(-1). however, its metabolite, ferulic acid ethyl ester, showed no antineoplastic activity in vitro.
WOS标题词	science & technology ; life sciences & biomedicine ; physical sciences
类目[WOS]	biochemical research methods ; chemistry, analytical
研究领域[WOS]	biochemistry & molecular biology ; chemistry
关键词[WOS]	performance liquid-chromatography ; mass-spectrometry ; ferulic acid ; cnidium-officinale ; angelica-sinensis ; tetramethylpyrazine ; hydroxyphthalides ; pregnenolone ; ingredients ; components
收录类别	SCI
原文出处	true
语种	英语
WOS记录号	WOS:000240281400010
公开日期	2010-11-30
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/97285]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Dalian Univ Technol, Sch Environm & Biol Sci & Technol, Dalian 116024, Peoples R China
推荐引用方式 GB/T 7714	Kong, Liang,Yu, Zhiyuan,Bao, Yongming,et al. Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS[J]. analytical and bioanalytical chemistry,2006,386(2):264-274.
APA	Kong, Liang,Yu, Zhiyuan,Bao, Yongming,Su, Xingye,Zou, Hanfa,&Li, Xin.(2006).Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS.analytical and bioanalytical chemistry,386(2),264-274.
MLA	Kong, Liang,et al."Screening and analysis of an antineoplastic compound in Rhizoma Chuanxiong by means of in vitro metabolism and HPLC-MS".analytical and bioanalytical chemistry 386.2(2006):264-274.