Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine

doi:10.1021/acsomega.8b00827

CORC > 武汉植物园 > 中国科学院武汉植物园

	Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine
	Vimolmangkang, Sornkanok 3,5; De-Eknamkul, Wanchai 3,5; Rani, Dolly 3,5; Han, Yuepeng 2; Chansilpa, N. Nopchai 1; Kobtrakul, Khwanlada 3,5; Meelaph, Thitirat 4,5
刊名	ACS OMEGA
	2018-08-01
卷号	3 期号:8 页码:8794-8802
ISSN号	2470-1343
DOI	10.1021/acsomega.8b00827
英文摘要	Lotus (Nelumbo nucifera Gaertn) contains various bioactive compounds, with benzylisoquinoline alkaloids (BIAs) as one of the major groups. The biosynthetic pathways of two major bioactive BIAs in this plant, nuciferine and N-nornuciferine, are still not clear. Therefore, several genes related to BIA biosynthesis were searched from the lotus database to study the role of key genes in regulating these pathways. In this study, the expression profiles of NCS, CNMT, 6OMT, CYP80G2, and WRKY TFs were investigated in mechanically wounded lotus leaves. It was found that the accumulation of nuciferine and N-nornuciferine significantly increased in the mechanically wounded lotus leaves in accordance with the relative expression of putative CYP80G2 and one WRKY transcription factor (NNU_24385), with the coregulation of CNMT. Furthermore, the role of methyltransferase-related. genes in this study suggested that methylation of the isoquinoline nucleus to yield a methylated-BIA structure may occur at the N position before the O position. Altogether, this study provides improved understanding of the genes regulating BIA biosynthesis under stressed conditions, which could lead to improvements in BIA production from the commercial lotus.
资助项目	Chulalongkorn University Fund ; National Science and Technology Development Agency[SCH-NR2016-091]
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000444182900017
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5839]
专题	中国科学院武汉植物园
通讯作者	Vimolmangkang, Sornkanok
作者单位	1.Rajamangala Univ Technol Tawan Ok, Fac Agr & Nat Resources, Chon Buri 20110, Thailand 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 3.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Pharmacognosy & Pharmaceut Bot, Bangkok 10330, Thailand 4.Chulalongkorn Univ, Fac Pharmaceut Sci, Dept Biochem & Microbiol, Bangkok 10330, Thailand 5.Chulalongkorn Univ, Fac Pharmaceut Sci, Res Unit Nat Prod Biotechnol, Bangkok 10330, Thailand
推荐引用方式 GB/T 7714	Vimolmangkang, Sornkanok,De-Eknamkul, Wanchai,Rani, Dolly,et al. Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine[J]. ACS OMEGA,2018,3(8):8794-8802.
APA	Vimolmangkang, Sornkanok.,De-Eknamkul, Wanchai.,Rani, Dolly.,Han, Yuepeng.,Chansilpa, N. Nopchai.,...&Meelaph, Thitirat.(2018).Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine.ACS OMEGA,3(8),8794-8802.
MLA	Vimolmangkang, Sornkanok,et al."Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine".ACS OMEGA 3.8(2018):8794-8802.