The contribution of TRPV1 channel to 20-HETE-Aggravated ischemic neuronal injury

doi:10.1016/j.prostaglandins.2018.07.001

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	The contribution of TRPV1 channel to 20-HETE-Aggravated ischemic neuronal injury
	Munnuri, Sailu 4; Yang, Zeng-Jin 1; Zhang, Xiaofan 1,2,3; El Demerdash, Nagat 1; Falck, John R.4; Koehler, Raymond C.1
刊名	PROSTAGLANDINS & OTHER LIPID MEDIATORS
	2018-07-01
卷号	137 页码:63-68
关键词	20-HETE TRPV1 Oxygen-glucose deprivation Hypoxic-ischemic injury Neonatal
ISSN号	1098-8823
DOI	10.1016/j.prostaglandins.2018.07.001
英文摘要	20-Hydroxyeicosatetraenoic acid (20-HETE), a cytochrome P450 (CYP) 4A/4F-derived metabolite of arachidonic acid, directly contributes to ischemic neuronal injury. However, little is known about mediators of 20-HETE neurotoxicity after ischemia. Here, we focus on the role of transient receptor potential cation channel subfamily V member 1 (TRPV1) in 20-HETE-induced neurotoxicity. Our results showed that TRPV1 and CYP4A irnmunoreactivity were colocalized in neurons. TRPV1 inhibition attenuated 20-HETE mimetic 20-5,14-HEDGE-induced reactive oxygen species (ROS) production and neuronal injury in cultured neurons and protected ischemic neurons in vitro and in vivo. TRPV1 inhibition in combination with 20-HETE synthesis inhibitor HET0016 did not produce additional protective effects. Furthermore, TRPV1 genetic inhibition and NADPH oxidase inhibitor gp9lds-dat each attenuated ROS production to a similar extent. However, combined treatment did not achieve additional reduction. Therefore, we conclude that TRPV1 channels are involved in 20-HETE's ROS generation and neurotoxicity after ischemia.
资助项目	Chinese Scholarship Council ; National Institutes of Health (NIH)[HL139793] ; Robert A. Welch Foundation[I-0011] ; NIH[NS060703] ; NIH[NS067525] ; Stimulating and Advancing ACCM Research (StAAR) grant from the Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000440957900008
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5544]
专题	中国科学院武汉植物园
通讯作者	Yang, Zeng-Jin
作者单位	1.Johns Hopkins Univ, Dept Anesthesiol Crit Care Med, 720 Rutland Ave,Traylor 804, Baltimore, MD 21205 USA 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Univ Texas Southwestern Med Ctr Dallas, Dept Biochem, Dallas, TX 75390 USA
推荐引用方式 GB/T 7714	Munnuri, Sailu,Yang, Zeng-Jin,Zhang, Xiaofan,et al. The contribution of TRPV1 channel to 20-HETE-Aggravated ischemic neuronal injury[J]. PROSTAGLANDINS & OTHER LIPID MEDIATORS,2018,137:63-68.
APA	Munnuri, Sailu,Yang, Zeng-Jin,Zhang, Xiaofan,El Demerdash, Nagat,Falck, John R.,&Koehler, Raymond C..(2018).The contribution of TRPV1 channel to 20-HETE-Aggravated ischemic neuronal injury.PROSTAGLANDINS & OTHER LIPID MEDIATORS,137,63-68.
MLA	Munnuri, Sailu,et al."The contribution of TRPV1 channel to 20-HETE-Aggravated ischemic neuronal injury".PROSTAGLANDINS & OTHER LIPID MEDIATORS 137(2018):63-68.