Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii

doi:10.3389/fpls.2018.00676

CORC > 武汉植物园 > 中国科学院武汉植物园

	Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii
	Zhang, Yuxiang 1; Zhang, Weiqing 1; Zhong, Huan 1; Lu, Hui 1; Deng, Xuan 2; Huang, Kaiyao 2; Duanmu, Deqiang 1
刊名	FRONTIERS IN PLANT SCIENCE
	2018-05-23
卷号	9 页码:13
关键词	PCYA1 bilin POR chlorophyll biosynthesis algae photoacclimation
ISSN号	1664-462X
DOI	10.3389/fpls.2018.00676
英文摘要	Bilins are linear tetrapyrroles commonly used as chromophores of phycobiliproteins and phytochromes for light-harvesting or light-sensing in photosynthetic organisms. Many eukaryotic algae lack both phycobiliproteins and phytochromes, but retain the bilin biosynthetic enzymes including heme oxygenase (HO/HMOX) and ferredoxin-dependent biliverdin reductase (FDBR). Previous studies on Chlamydomonas reinhardtii heme oxygenase mutant (hmox1) have shown that bilins are not only essential retrograde signals to mitigate oxidative stress during diurnal dark-to-light transitions, they are also required for chlorophyll accumulation and maintenance of a functional photosynthetic apparatus in the light. However, the underlying mechanism of bilin-mediated regulation of chlorophyll biosynthesis is unclear. In this study, Chlamydomonas phycocyanobilin:ferredoxin oxidoreductase PCYA1 FDBR domain was found to specifically interact with the rate-limiting chlorophyll biosynthetic enzyme LPOR (light-dependent protochlorophyllide oxidoreductase). PCYA1 is partially associated with chloroplast envelope membrane, consistent with the observed export of bilin from chloroplast to cytosol by cytosolic expression of a bilin-binding reporter protein in Chlamydomonas. Both the pcya1-1 mutant with the carboxyl-terminal extension of PCYA1 eliminated and efficient knockdown of PCYA1 expression by artificial microRNA exhibited no significant impact on algal phototrophic growth and photosynthetic proteins accumulation, indicating that the conserved FDBR domain is sufficient and minimally required for bilin biosynthesis and functioning. Taken together, these studies provide novel insights into the regulatory role of PCYA1 in chlorophyll biosynthesis via interaction with key Chl biosynthetic enzyme.
资助项目	National Natural Science Foundation of China[31570233] ; Huazhong Agricultural University Scientific and Technological Self-innovation Foundation[2014RC018] ; Fundamental Research Funds for the Central Universities[2662015PY171]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000432772000001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5377]
专题	中国科学院武汉植物园
通讯作者	Duanmu, Deqiang
作者单位	1.Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yuxiang,Zhang, Weiqing,Zhong, Huan,et al. Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii[J]. FRONTIERS IN PLANT SCIENCE,2018,9:13.
APA	Zhang, Yuxiang.,Zhang, Weiqing.,Zhong, Huan.,Lu, Hui.,Deng, Xuan.,...&Duanmu, Deqiang.(2018).Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii.FRONTIERS IN PLANT SCIENCE,9,13.
MLA	Zhang, Yuxiang,et al."Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii".FRONTIERS IN PLANT SCIENCE 9(2018):13.