Ac102 Participates in Nuclear Actin Polymerization by Modulating BV/ODV-C42 Ubiquitination during Autographa californica Multiple Nucleopolyhedrovirus Infection

doi:10.1128/JVI.00005-18

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	Ac102 Participates in Nuclear Actin Polymerization by Modulating BV/ODV-C42 Ubiquitination during Autographa californica Multiple Nucleopolyhedrovirus Infection
	Chen, Xinwen 2; Zhang, Yongli 1,2; Hu, Xue 2; Mu, Jingfang 2; Hu, Yangyang 2; Zhou, Yuan 2; Zhao, He 2; Wu, Chunchen 2; Pei, Rongjuan 2; Chen, Jizheng 2
刊名	JOURNAL OF VIROLOGY
	2018-06-01
卷号	92 期号:12 页码:14
关键词	Ac102 AcMNPV BV/ODV-C42 P78/83 actin polymerization ubiquitination
ISSN号	0022-538X
DOI	10.1128/JVI.00005-18
英文摘要	As a virus-encoded actin nucleation promoting factor (NPF), P78/83 induces actin polymerization to assist in Autographa californica multiple nucleopolyhedrovirus (AcMNPV) propagation. According to our previous study, although P78/83 actively undergoes ubiquitin-independent proteasomal degradation, AcMNPV encodes budded virus/occlusion derived virus (BV/ODV)-C42 (C42), which allows P78/83 to function as a stable NPF by inhibiting its degradation during viral infection. However, whether there are other viral proteins involved in regulating P78/83-induced actin polymerization has yet to be determined. In this study, we found that Ac102, an essential viral gene product previously reported to play a key role in mediating the nuclear accumulation of actin during AcMNPV infection, is a novel regulator of P78/83-induced actin polymerization. By characterizing an ac102 knockout bacmid, we demonstrated that Ac102 participates in regulating nuclear actin polymerization as well as the morphogenesis and distribution of capsid structures in the nucleus. These regulatory effects are heavily dependent on an interaction between Ac102 and C42. Further investigation revealed that Ac102 binds to C42 to suppress K48-linked ubiquitination of C42, which decreases C42 proteasomal degradation and consequently allows P78/83 to function as a stable NPF to induce actin polymerization. Thus, Ac102 and C42 form a regulatory cascade to control viral NPF activity, representing a sophisticated mechanism for AcMNPV to orchestrate actin polymerization in both a ubiquitin-dependent and ubiquitin-independent manner. IMPORTANCE Actin is one of the most functionally important proteins in eukaryotic cells. Morphologically, actin can be found in two forms: a monomeric form called globular actin (G-actin) and a polymeric form called filamentous actin (F-actin). G-actin can polymerize to form F-actin, and nucleation promoting factor (NPF) is the initiator of this process. Many viral pathogens harness the host actin polymerization machinery to assist in virus propagation. Autographa californica multiple nucleopolyhedrovirus (AcMNPV) induces actin polymerization in host cells. P78/83, a viral NPF, is responsible for this process. Previously, we identified that BV/ODV-C42 (C42) binds to P78/83 and protects it from degradation. In this report, we determined that another viral protein, Ac102, is involved in modulating C42 ubiquitination and, consequently, ensures P78/83 activity as an NPF to initiate actin polymerization. This regulatory cascade represents a novel mechanism by which a virus can harness the cellular actin cytoskeleton to assist in viral propagation.
资助项目	National Natural Science Foundation of China[31770170] ; National Natural Science Foundation of China[31470261] ; National Natural Science Foundation of China[31621061]
WOS研究方向	Virology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000433416900001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/5323]
专题	中国科学院武汉植物园
通讯作者	Chen, Xinwen; Wang, Yun
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xinwen,Zhang, Yongli,Hu, Xue,et al. Ac102 Participates in Nuclear Actin Polymerization by Modulating BV/ODV-C42 Ubiquitination during Autographa californica Multiple Nucleopolyhedrovirus Infection[J]. JOURNAL OF VIROLOGY,2018,92(12):14.
APA	Chen, Xinwen.,Zhang, Yongli.,Hu, Xue.,Mu, Jingfang.,Hu, Yangyang.,...&Wang, Yun.(2018).Ac102 Participates in Nuclear Actin Polymerization by Modulating BV/ODV-C42 Ubiquitination during Autographa californica Multiple Nucleopolyhedrovirus Infection.JOURNAL OF VIROLOGY,92(12),14.
MLA	Chen, Xinwen,et al."Ac102 Participates in Nuclear Actin Polymerization by Modulating BV/ODV-C42 Ubiquitination during Autographa californica Multiple Nucleopolyhedrovirus Infection".JOURNAL OF VIROLOGY 92.12(2018):14.