Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus

doi:10.1016/j.virol.2017.09.019

CORC > 武汉植物园 > 中国科学院武汉植物园

	Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus
	Zhang, Jie 1,2; Fang, Qin 1; Zhang, Fuxian 1; Guo, Hong 1; Chen, Qingxiu 1
刊名	VIROLOGY
	2018
卷号	513 页码:195-207
关键词	DsRNA virus Reovirus Aquareovirus Cell entry Quantum dot Caveolae/raft-mediated endocytosis
ISSN号	0042-6822
DOI	10.1016/j.virol.2017.09.019
英文摘要	Grass carp reovirus (GCRV), a member of the Aquareovirus genus in the Reoviridae family, is considered the most pathogenic aquareovirus. However, its productive viral entry pathways remain largely unclear. Using a combination of quantum dot (QD)-based live-virus tracking and biochemical assays, we found that extraction of cellular membrane cholesterol with methyl-beta-cyclodextrin (M beta CD) and nystatin strongly inhibited the internalization of GCRVs, and supplementation with cholesterol restored viral infection. In addition, the entry of the virus was restrained by genistein, an inhibitor known to block caveolar endocytosis. Subsequent real-time tracking experiments revealed that the QD-labeled GCRV particles were colocalized with caveolin-1, and transfection of cells with dominant-negative mutant (caveolin-1 Y14F) significantly reduced GCRV infection. In contrast, no effects on virus infection were detected when the clathrin-mediated endocytosis or the macro-pinocytosis inhibitors were used. Our results collectively suggest that aquareoviruses can use caveolae/raft-mediated endocytosis as the primary entry pathway to initiate productive infection.
资助项目	National Natural Science Foundation of China[31372565] ; National Natural Science Foundation of China[31400139] ; National Natural Science Foundation of China[31370190] ; National Natural Science Foundation of China[31672693] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2013FB05]
WOS研究方向	Virology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000417670400022
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/4418]
专题	中国科学院武汉植物园
通讯作者	Fang, Qin
作者单位	1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jie,Fang, Qin,Zhang, Fuxian,et al. Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus[J]. VIROLOGY,2018,513:195-207.
APA	Zhang, Jie,Fang, Qin,Zhang, Fuxian,Guo, Hong,&Chen, Qingxiu.(2018).Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus.VIROLOGY,513,195-207.
MLA	Zhang, Jie,et al."Identification of the caveolae/raft-mediated endocytosis as the primary entry pathway for aquareovirus".VIROLOGY 513(2018):195-207.