The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response

doi:10.1016/j.bbrc.2017.04.066

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	The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response
	Li, Lin-Mao 2,3; Lu, Shi-You 1,2; Li, Rong-Jun 1,2
刊名	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
	2017-05-27
卷号	487 期号:2 页码:362-367
关键词	CER9 HRD1 ERAD Heat stress response Cytosolic protein response Unfolded protein response
ISSN号	0006-291X
DOI	10.1016/j.bbrc.2017.04.066
英文摘要	The Cytosolic Protein Response (CPR) in the cytosol and the Unfolded Protein Response (UPR) and ER associated degradation (ERAD) in the endoplasmic reticulum are major pathways of the cellular proteostasis network. However, despite years of effort, how these protein quality control systems coordinated in vivo remains largely unknown, particularly in plants. In this study, the roles of two evolutionarily conserved ERAD pathways (DOA10 and HRD1) in heat stress response were investigated through reverse genetic approaches in Arabidopsis. Phenotypic analysis of the mutants showed that the two ERAD pathways additively play negative roles in heat tolerance, which was demonstrated by higher survival rate and lower electrolyte leakage in the loss of function mutants compared to the wild type plants. Importantly, gene expression analysis revealed that the mutant plants showed elevated transcriptional regulation of several downstream genes, including those encoding CPR and UPR marker genes, under both basal and heat stress conditions. Finally, multiple components of ERAD genes exhibited rapid response to increasing temperature. Taken together, our data not only unravels key insights into the crosstalk between different protein quality control processes, but also provides candidate genes to genetically improve plant heat tolerance in the future. (C) 2017 Elsevier Inc. All rights reserved.
资助项目	Natural Science Foundation of China[31400276] ; Natural Science Foundation of China[31370338] ; Natural Science Foundation of China[31570186]
WOS研究方向	Biochemistry & Molecular Biology ; Biophysics
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000401683700028
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1366]
专题	中国科学院武汉植物园
通讯作者	Li, Rong-Jun
作者单位	1.Chinese Acad Sci, Sinoafrica Joint Res Ctr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Li, Lin-Mao,Lu, Shi-You,Li, Rong-Jun. The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2017,487(2):362-367.
APA	Li, Lin-Mao,Lu, Shi-You,&Li, Rong-Jun.(2017).The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,487(2),362-367.
MLA	Li, Lin-Mao,et al."The Arabidopsis endoplasmic reticulum associated degradation pathways are involved in the regulation of heat stress response".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 487.2(2017):362-367.