Low genetic diversity and functional constraint of miRNA genes participating pollen-pistil interaction in rice

doi:10.1007/s11103-017-0638-0

CORC > 武汉植物园 > 中国科学院武汉植物园

	Low genetic diversity and functional constraint of miRNA genes participating pollen-pistil interaction in rice
	Shi, Tao 1; Wang, Kun 1,3; Wang, Xin 1; Li, Ming 1; Yang, Pingfang 1,2
刊名	PLANT MOLECULAR BIOLOGY
	2017-09-01
卷号	95 期号:1-2 页码:89-98
关键词	MicroRNA Oryza sativa Evolution Pistil-pollination interaction
ISSN号	0167-4412
DOI	10.1007/s11103-017-0638-0
英文摘要	Pollen-pistil interaction is an essential reproductive process for all flowering plants. While microRNAs (miRNAs) are important noncoding small RNAs that regulate mRNA levels in eukaryotic cells, there is little knowledge about which miRNAs involved in the early stages of pollen-pistil interaction in rice and how they evolve under this conserved process. In this study, we sequenced the small RNAs in rice from unpollinated pistil (R0), pistil from 5 min and 15 min after pollination, respectively, to identify known and novel miRNAs that are involved in this process. By comparing the corresponding mRNA-seq dataset, we identified a group of miRNAs with strong negative expression pattern with their target genes. Further investigation of all miRNA loci (MIRNAs) across 1083 public rice accessions revealed significantly reduced genetic diversity in MIRNAs with strong negative expression of their targets when comparing to those with little or no impact on targets during pollen-pistil interaction. Annotation of targets suggested that those MIRNAs with strong impact on targets were pronounced in cell wall related processes such as xylan metabolism. Additionally, plant conserved miRNAs, such as those with functions in gibberellic acid, auxin and nitrate signaling, were also with strong negative expression of their targets. Overall, our analyses identified key miRNAs participating pollen-pistil interaction and their evolutionary patterns in rice, which can facilitate the understanding of molecular mechanisms associated with seed setting.
资助项目	National Natural Science Foundation of China[31671775] ; National Natural Science Foundation of China[31100230] ; National Key Research and Development Program of China[2016YFD0100904] ; Open Research Fund of State Key Laboratory of Hybrid Rice, Wuhan University[KF201306]
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000410475500006
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/1316]
专题	中国科学院武汉植物园
通讯作者	Shi, Tao; Yang, Pingfang
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan, Hubei, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Shi, Tao,Wang, Kun,Wang, Xin,et al. Low genetic diversity and functional constraint of miRNA genes participating pollen-pistil interaction in rice[J]. PLANT MOLECULAR BIOLOGY,2017,95(1-2):89-98.
APA	Shi, Tao,Wang, Kun,Wang, Xin,Li, Ming,&Yang, Pingfang.(2017).Low genetic diversity and functional constraint of miRNA genes participating pollen-pistil interaction in rice.PLANT MOLECULAR BIOLOGY,95(1-2),89-98.
MLA	Shi, Tao,et al."Low genetic diversity and functional constraint of miRNA genes participating pollen-pistil interaction in rice".PLANT MOLECULAR BIOLOGY 95.1-2(2017):89-98.