Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange

doi:10.1016/j.scienta.2010.10.007

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	Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange
	Wang, Yong 1; Li, Jing 2; Yang, Jing 1; Xia, Renxue 1
刊名	SCIENTIA HORTICULTURAE
	2011-01-10
卷号	127 期号:3 页码:267-274
关键词	Satsuma mandarin Navel orange Carotenoid Lycopene cyclase Real-time PCR HPLC
ISSN号	0304-4238
DOI	10.1016/j.scienta.2010.10.007
英文摘要	Lycopene beta-cyclase (LCYb) and lycopene epsilon-cyclase (LCYe) are believed to be crucial genes to lycopene cyclization and downstream carotenoid accumulation. To illuminate the gene expression profiles and their regulation on downstream carotenoids, expression profiles of these two genes and contents of several corresponding carotenoids were determined by real-time PCR and HPLC, respectively, in relative shorter intervals during breaker and maturation stage of Guoqing No. 1 satsuma mandarin (Citrus unshiu Marcow) and Cara Cara navel orange (Citrus sinensis Osbeck). Data showed that the expression profiles of these two cyclase genes and their relative expression levels between the peels and the pulps at the same stage all varied with cultivars. But in general, accumulation of lutein and beta-carotene experienced a similar tendency in both cultivars and were up-regulated by expression of LCYb and LCYe in spite of poor correlation in the pulps. Meanwhile, lycopene abundance was negatively correlated with the expression of LCYe as expected, but hardly correlated with LCYb in the pulps of Cara Cara navel orange, indicating that LCYe might exert a more prominent influence on lycopene cyclization and downstream carotenoids formation. Results of carotenoids accumulation suggest that the biosynthetic process of carotenoids would shift from upstream beta, epsilon- or beta, beta-carotenoids such as lutein and beta-carotene to downstream beta, beta-carotenoids during fruit development. (c) 2010 Elsevier B.V. All rights reserved.
资助项目	National Public Benefit (Agriculture) Research Foundation[nyhyzx07-023]
WOS研究方向	Agriculture
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000286712300013
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/894]
专题	中国科学院武汉植物园
通讯作者	Xia, Renxue
作者单位	1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Subject Grp Mol Breeding Fruit Trees, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yong,Li, Jing,Yang, Jing,et al. Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange[J]. SCIENTIA HORTICULTURAE,2011,127(3):267-274.
APA	Wang, Yong,Li, Jing,Yang, Jing,&Xia, Renxue.(2011).Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange.SCIENTIA HORTICULTURAE,127(3),267-274.
MLA	Wang, Yong,et al."Expression of lycopene cyclase genes and their regulation on downstream carotenoids during fruit maturation of Guoqing No. 1 Satsuma mandarin and Cara Cara navel orange".SCIENTIA HORTICULTURAE 127.3(2011):267-274.