Testing four barcoding markers for species identification of Potamogetonaceae

doi:10.1111/j.1759-6831.2011.00131.x

CORC > 武汉植物园 > 中国科学院武汉植物园

	Testing four barcoding markers for species identification of Potamogetonaceae
	Du, Zhi-Yuan 1,2; Qimike, Alitong 3; Yang, Chun-Feng 1,2; Chen, Jin-Ming 1,2; Wang, Qing-Feng 1,2
刊名	JOURNAL OF SYSTEMATICS AND EVOLUTION
	2011-05-01
卷号	49 期号:3 页码:246-251
关键词	DNA barcoding internal transcribed spacer (ITS) matK Potamogetonaceae rbcL trnH-psbA
ISSN号	1674-4918
DOI	10.1111/j.1759-6831.2011.00131.x
英文摘要	The pondweeds (Potamogetonaceae) are among the most important plant groups in the aquatic environment. Owing to their high morphological and ecological diversity, species identification of this aquatic family remains problematic. DNA barcoding involves sequencing a standard DNA region and has been shown to be a powerful tool for species identification. In the present study, we tested four barcoding markers (rbcL, matK, internal transcribed spacer (ITS), and trnH-psbA) in 15 Potamogeton species and two Stuckenia species, representing most species of the Potamogetonaceae in China. The results show that all four regions can distinguish and support the newly proposed genera of Stuckenia from Potamogeton. Using ITS and trnH-psbA, significant interspecific genetic variability was shown. However, intraspecific genetic variability of trnH-psbA is high and so it is not suitable for barcoding in Potamogetonaceae. The ITS and matK regions showed good discrimination. However, matK was not easy to sequence using universal primers. The best performing single locus was ITS, making it a potentially useful DNA barcode in Potamogetonaceae.
资助项目	Chinese Academy of Sciences[KSCX2-YW-Z-0805] ; Chinese Academy of Sciences[2009-LSF-GBOWS-01] ; National Natural Science Foundation of China[30970195]
WOS研究方向	Plant Sciences
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000291236500010
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/871]
专题	中国科学院武汉植物园
通讯作者	Wang, Qing-Feng
作者单位	1.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Xinjiang Bazhou Normal Coll, Kuerle 841000, Peoples R China
推荐引用方式 GB/T 7714	Du, Zhi-Yuan,Qimike, Alitong,Yang, Chun-Feng,et al. Testing four barcoding markers for species identification of Potamogetonaceae[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2011,49(3):246-251.
APA	Du, Zhi-Yuan,Qimike, Alitong,Yang, Chun-Feng,Chen, Jin-Ming,&Wang, Qing-Feng.(2011).Testing four barcoding markers for species identification of Potamogetonaceae.JOURNAL OF SYSTEMATICS AND EVOLUTION,49(3),246-251.
MLA	Du, Zhi-Yuan,et al."Testing four barcoding markers for species identification of Potamogetonaceae".JOURNAL OF SYSTEMATICS AND EVOLUTION 49.3(2011):246-251.