Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach

doi:10.1002/pmic.201000598

CORC > 武汉植物园 > 中国科学院武汉植物园

	Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach
	He, Dongli 1; Han, Chao 1,2; Yao, Jialing 3; Shen, Shihua 4; Yang, Pingfang 1
刊名	PROTEOMICS
	2011-07-01
卷号	11 期号:13 页码:2693-2713
关键词	Germination Metabolic pathway Plant proteomics Rice Seed
ISSN号	1615-9853
DOI	10.1002/pmic.201000598
英文摘要	Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale data within the overall physiological scheme of an organism. It is an efficient way to predict metabolic phenotype or regulatory style. We did protein profiling in the germinating rice seeds through 1-DE via LC MS/MS proteomic shotgun strategy. In total, 673 proteins were identified, and could be sorted into 14 functional groups. The largest group was metabolism related. The metabolic proteins were integrated into different metabolic pathways to show the style of reserves mobilization and precursor preparation during the germination. Analysis of the regulatory proteins indicated that regulation of redox homeostasis and gene expression also play important roles for the rice seed germination. Although transcription is unnecessary for the germination, it could ensure the rapidity and uniformity of germination. On the contrary, translation with the stored mRNA is required for the germination. This study will help us to further understand the metabolic style, regulation of redox homeostasis, and gene expression during rice seed germination.
资助项目	Chinese Academy of Sciences
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000293074500011
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/863]
专题	中国科学院武汉植物园
通讯作者	Yang, Pingfang
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Huazhong Agr Univ, Coll Life Sci, Wuhan, Peoples R China 4.Chinese Acad Sci, Inst Bot, Res & Dev Ctr Energy Plants, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Dongli,Han, Chao,Yao, Jialing,et al. Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach[J]. PROTEOMICS,2011,11(13):2693-2713.
APA	He, Dongli,Han, Chao,Yao, Jialing,Shen, Shihua,&Yang, Pingfang.(2011).Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach.PROTEOMICS,11(13),2693-2713.
MLA	He, Dongli,et al."Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach".PROTEOMICS 11.13(2011):2693-2713.