Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes

doi:10.1093/jxb/err130

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	Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes
	Chan, Zhulong 2; Grumet, Rebecca 1; Loescher, Wayne 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2011-10-01
卷号	62 期号:14 页码:4787-4803
关键词	Abscisic acid cell wall mannitol mannose-6-phosphate reductase osmoprotectant redox salt tolerance
ISSN号	0022-0957
DOI	10.1093/jxb/err130
英文摘要	Mannitol is a putative osmoprotectant contributing to salt tolerance in several species. Arabidopsis plants transformed with the mannose-6-phosphate reductase (M6PR) gene from celery were dramatically more salt tolerant (at 100 mM NaCl) as exhibited by reduced salt injury, less inhibition of vegetative growth, and increased seed production relative to the wild type (WT). When treated with 200 mM NaCl, transformants produced no seeds, but did bolt, and exhibited less chlorosis/necrosis and greater survival and dry weights than the WT. Without salt there were no M6PR effects on growth or phenotype, but expression levels of 2272 genes were altered. Many fewer differences (1039) were observed between M6PR and WT plants in the presence of salt, suggesting that M6PR pre-conditioned the plants to stress. Previous work suggested that mannitol is an osmoprotectant, but mannitol levels are invariably quite low, perhaps inadequate for osmoprotectant effects. In this study, transcriptome analysis reveals that the M6PR transgene activated the downstream abscisic acid (ABA) pathway by up-regulation of ABA receptor genes (PYL4, PYL5, and PYL6) and down-regulation of protein phosphatase 2C genes (ABI1 and ABI2). In the M6PR transgenic lines there were also increases in transcripts related to redox and cell wall-strengthening pathways. These data indicate that mannitol-enhanced stress tolerance is due at least in part to increased expression of a variety of stress-inducible genes.
资助项目	USDA-BRAG[2005-39454-16516]
WOS研究方向	Plant Sciences
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000295983800007
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/841]
专题	中国科学院武汉植物园
通讯作者	Loescher, Wayne
作者单位	1.Michigan State Univ, PSSB, Grad Program Plant Breeding Genet & Biotechnol, E Lansing, MI 48864 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Chan, Zhulong,Grumet, Rebecca,Loescher, Wayne. Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes[J]. JOURNAL OF EXPERIMENTAL BOTANY,2011,62(14):4787-4803.
APA	Chan, Zhulong,Grumet, Rebecca,&Loescher, Wayne.(2011).Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes.JOURNAL OF EXPERIMENTAL BOTANY,62(14),4787-4803.
MLA	Chan, Zhulong,et al."Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes".JOURNAL OF EXPERIMENTAL BOTANY 62.14(2011):4787-4803.