MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS

CORC > 武汉植物园 > 中国科学院武汉植物园

	MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS
	Li, Zuozhou; Li, Wei; Chen, Yuanyuan; Han, Qingxiang
刊名	AMERICAN JOURNAL OF BOTANY
	2012
英文摘要	Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species.
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/776]
专题	中国科学院武汉植物园
推荐引用方式 GB/T 7714	Li, Zuozhou,Li, Wei,Chen, Yuanyuan,et al. MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS[J]. AMERICAN JOURNAL OF BOTANY,2012.
APA	Li, Zuozhou,Li, Wei,Chen, Yuanyuan,&Han, Qingxiang.(2012).MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS.AMERICAN JOURNAL OF BOTANY.
MLA	Li, Zuozhou,et al."MICROSATELLITE PRIMERS IN THE ENDANGERED QUILLWORT ISOETES HYPSOPHILA (ISOETACEAE) AND CROSS-AMPLIFICATION IN I. SINENSIS".AMERICAN JOURNAL OF BOTANY (2012).