Identification of cold-inducible microRNAs in grapevine

doi:10.3389/fpls.2015.00595

CORC > 武汉植物园 > 中国科学院武汉植物园

	Identification of cold-inducible microRNAs in grapevine
	Xin, Haiping 2,3; Sun, Xiaoming 2,3,4; Fan, Gaotao 1; Su, Lingye 3,4; Wang, Wanjun 1; Liang, Zhenchang 3; Li, Shaohua 3
刊名	FRONTIERS IN PLANT SCIENCE
	2015-08-04
卷号	6 页码:13
关键词	Vitis vinifera microRNA cold-inducible abiotic stress Solexa
ISSN号	1664-462X
DOI	10.3389/fpls.2015.00595
英文摘要	Low temperature is one of the most important environmental factors that limits the geographical distribution and productivity of grapevine. However, the molecular mechanisms on how grapevine responds to cold stress remains to be elucidated. MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs that play an essential role during plant development and stress responses. Although miRNAs and their targets have been identified in several Vitis species, their participation during cold accumulation in grapevine remains unknown. In this study, two small RNA libraries were generated from micropropagated 'Muscat Hamburg' (V. vinifera) plantlets under normal and low temperatures (4 degrees C). A total of 163 known miRNAs and 67 putative novel miRNAs were detected from two small RNA libraries by Solexa sequencing. Forty-four cold-inducible miRNAs were identified through differentially expressed miRNAs (DEMs) analysis; among which, 13 belonged to upregulated DEMs while 31 belonged downregulated DEMs. The expression patterns of the 13 DEMs were verified by real-time RT-PCR analysis. The prediction of the target genes for DEMs indicated that miRNA may regulate transcription factors, including AP2, SBP, MYB, bHLH, GRAS, and bZIP under cold stress. The 5'-RLM RACE were conducted to verify the cleavage site of predicted targets. Seven predicted target genes for four known and three novel vvi-miRNAs showed specific cleavage sites corresponding to their miRNA complementary sequences. The expression pattern of these seven target genes revealed negative correlation with the expression level of the corresponding vvi-miRNAs. Our results indicated that a diverse set of miRNAs in V vinifera are cold-inducible and may play an important role in cold stress response.
资助项目	National Natural Science Foundation of China (NSFC)[31130047] ; National Natural Science Foundation of China (NSFC)[31471857] ; National Key Technology R&D Program of the Ministry of Science and Technology[2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS[2015281]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000359903300001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/222]
专题	中国科学院武汉植物园
通讯作者	Li, Shaohua
作者单位	1.Southwest Jiaotong Univ, Sch Life Sci & Engn, Dept Biol Engn, Chengdu, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Xin, Haiping,Sun, Xiaoming,Fan, Gaotao,et al. Identification of cold-inducible microRNAs in grapevine[J]. FRONTIERS IN PLANT SCIENCE,2015,6:13.
APA	Xin, Haiping.,Sun, Xiaoming.,Fan, Gaotao.,Su, Lingye.,Wang, Wanjun.,...&Li, Shaohua.(2015).Identification of cold-inducible microRNAs in grapevine.FRONTIERS IN PLANT SCIENCE,6,13.
MLA	Xin, Haiping,et al."Identification of cold-inducible microRNAs in grapevine".FRONTIERS IN PLANT SCIENCE 6(2015):13.