A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance

doi:10.1186/s12870-015-0598-y

CORC > 武汉植物园 > 中国科学院武汉植物园

	A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance
	Hu, Zhengrong 2,3,4; Chen, Liang 2,3; Fan, Jibiao 2,3,4; Hu, Longxing 2,3; Xie, Yan 2,3,4; Zhang, Yingzi 1; Lou, Yanhong 2,3; Nevo, Eviatar 5; Fu, Jinmin 2,3
刊名	BMC PLANT BIOLOGY
	2015-09-11
卷号	15 页码:22
ISSN号	1471-2229
DOI	10.1186/s12870-015-0598-y
英文摘要	Background: Cold stress is regarded as a key factor limiting widespread use for bermudagrass (Cynodon dactylon). Therefore, to improve cold tolerance for bermudagrass, it is urgent to understand molecular mechanisms of bermudagrass response to cold stress. However, our knowledge about the molecular responses of this species to cold stress is largely unknown. The objective of this study was to characterize the transcriptomic response to low temperature in bermudagrass by using RNA-Seq platform. Results: Ten cDNA libraries were generated from RNA samples of leaves from five different treatments in the cold-resistant (R) and the cold-sensitive (S) genotypes, including 4 degrees C cold acclimation (CA) for 24 h and 48 h, freezing (-5 degrees C) treatments for 4 h with or without prior CA, and controls. When subjected to cold acclimation, global gene expressions were initiated more quickly in the R genotype than those in the S genotype. The R genotype activated gene expression more effectively in response to freezing temperature after 48 h CA than the S genotype. The differentially expressed genes were identified as low temperature sensing and signaling-related genes, functional proteins and transcription factors, many of which were specifically or predominantly expressed in the R genotype under cold treatments, implying that these genes play important roles in the enhanced cold hardiness of bermudagrass. KEGG pathway enrichment analysis for DEGs revealed that photosynthesis, nitrogen metabolism and carbon fixation pathways play key roles in bermudagrass response to cold stress. Conclusions: The results of this study may contribute to our understanding the molecular mechanism underlying the responses of bermudagrass to cold stress, and also provide important clues for further study and in-depth characterization of cold-resistance breeding candidate genes in bermudagrass.
资助项目	China National Science Foundation (NSFC)[31272194] ; China National Science Foundation (NSFC)[31401915] ; China-Africa Center for Research and Education[SAJC201325] ; Hubei Province National Science Foundation Sciences[ZRY1326] ; Hubei Key Laboratory of Genetic Regulation and Integrative Biology[GRIB201307] ; outstanding young talent program of CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture[Y452341X01]
WOS研究方向	Plant Sciences
语种	英语
出版者	BIOMED CENTRAL LTD
WOS记录号	WOS:000361033200001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/208]
专题	中国科学院武汉植物园
通讯作者	Nevo, Eviatar
作者单位	1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Univ Haifa, Inst Evolut, IL-31905 Haifa, Israel
推荐引用方式 GB/T 7714	Hu, Zhengrong,Chen, Liang,Fan, Jibiao,et al. A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance[J]. BMC PLANT BIOLOGY,2015,15:22.
APA	Hu, Zhengrong.,Chen, Liang.,Fan, Jibiao.,Hu, Longxing.,Xie, Yan.,...&Fu, Jinmin.(2015).A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance.BMC PLANT BIOLOGY,15,22.
MLA	Hu, Zhengrong,et al."A transcriptomic analysis of bermudagrass (Cynodon dactylon) provides novel insights into the basis of low temperature tolerance".BMC PLANT BIOLOGY 15(2015):22.