Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS

doi:10.3390/molecules201219859

CORC > 武汉植物园 > 中国科学院武汉植物园

	Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS
	Zhu, Mingzhi 1,2; Dong, Xia 1; Guo, Mingquan 1,3
刊名	MOLECULES
	2015-12-01
卷号	20 期号:12 页码:22463-22475
关键词	Duchesnea indica phenolic profiling HPLC mass spectrometry
ISSN号	1420-3049
DOI	10.3390/molecules201219859
英文摘要	Duchesnea indica (D. indica) is an important traditional Chinese medicine, and has long been clinically used to treat cancer in Asian countries. It has been described previously as a rich source of phenolic compounds with a broad array of diversified structures, which are the major active ingredients. However, an accurate and complete phenolic profiling has not been determined yet. In the present work, the total phenolic compounds in crude extracts from D. indica were enriched and fractionated over a macroporous resin column, then identified by HPLC-ESI-MS/MS and ESI-IT-MS (ion trap MS). A total of 27 phenolic compounds were identified in D. indica, of which 21 compounds were identified for the first time. These 27 phenolic compounds encompassing four phenolic groups, including ellagitannins, ellagic acid and ellagic acid glycosides, hydroxybenzoic acid and hydroxycinnamic acid derivatives, and flavonols, were then successfully quantified using peak areas against those of the corresponding standards with good linearity (R-2 > 0.998) in the range of the tested concentrations. As a result, the contents of individual phenolic compounds varied from 6.69 mg per 100 g dry weight (DW) for ellagic acid to 71.36 mg per 100 g DW for brevifolin carboxylate. Not only did this study provide the first phenolic profiling of D. indica, but both the qualitative identification and the subsequent quantitative analysis of 27 phenolic compounds from D. indica should provide a good basis for future exploration of this valuable medicinal plant.
资助项目	Chinese Academy of Sciences[29Y429291a0129] ; Sino-Africa joint research project[SAJC201328]
WOS研究方向	Chemistry
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000367533400110
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/170]
专题	中国科学院武汉植物园
通讯作者	Guo, Mingquan
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Macau, Peoples R China 3.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Mingzhi,Dong, Xia,Guo, Mingquan. Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS[J]. MOLECULES,2015,20(12):22463-22475.
APA	Zhu, Mingzhi,Dong, Xia,&Guo, Mingquan.(2015).Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS.MOLECULES,20(12),22463-22475.
MLA	Zhu, Mingzhi,et al."Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS".MOLECULES 20.12(2015):22463-22475.