Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)

doi:10.1016/j.jprot.2015.10.010

CORC > 武汉植物园 > 中国科学院武汉植物园

	Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)
	Liu, Yanli 1; Chaturvedi, Palak 2; Fu, Jinlei 1; Cai, Qingqing 3; Weckwerth, Wolfram 2; Yang, Pingfang 1
刊名	JOURNAL OF PROTEOMICS
	2016-01-10
卷号	131 页码:61-70
关键词	Lotus Cotyledons Callus induction Quantitative proteomics
ISSN号	1874-3919
DOI	10.1016/j.jprot.2015.10.010
英文摘要	Lotus is an aquatic plant with high nutritional, ornamental and medical values. Its callus formation is crucial for germplasm innovation by genetic transformation. In this study, embryogenic callus was successfully induced on appropriate medium using cotyledons at 12 days after pollination as explants. To dissect cellular dedifferentiation and callus formation processes at the proteome level, cotyledons before and tissues from 10 to 20 days after induction were sampled for shotgun proteomic analysis. By applying multivariate statistics 91 proteins were detected as differentially regulated, and sorted into 6 functional groups according to MapMan ontology analysis. Most of these proteins were implicated in various metabolisms, demonstrating that plant cells underwent metabolism reprogramming during callus induction. 143% proteins were associated with stress and redox, indicating that the detached explants were subjected to a variety of stresses; 13.2% were cell and cell wall-related proteins, suggesting that these proteins played important roles in rapid cell division and proliferation. Some proteins were further evaluated at the mRNA levels by quantitative reverse transcription PCR analysis. In conclusion, the results contributed to further deciphering of molecular processes of cellular dedifferentiation and callus formation, and provided a reference data set for the establishment of transgenic transformation in lotus. (C) 2015 Elsevier B.V. All rights reserved.
资助项目	Knowledge Innovation Project[Y455421Z02]
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000366540200007
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/136]
专题	中国科学院武汉植物园
通讯作者	Yang, Pingfang
作者单位	1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Moshan 430074, Peoples R China 2.Univ Vienna, Dept Ecogen & Syst Biol, A-1090 Vienna, Austria 3.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yanli,Chaturvedi, Palak,Fu, Jinlei,et al. Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)[J]. JOURNAL OF PROTEOMICS,2016,131:61-70.
APA	Liu, Yanli,Chaturvedi, Palak,Fu, Jinlei,Cai, Qingqing,Weckwerth, Wolfram,&Yang, Pingfang.(2016).Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian).JOURNAL OF PROTEOMICS,131,61-70.
MLA	Liu, Yanli,et al."Induction and quantitative proteomic analysis of cell dedifferentiation during callus formation of lotus (Nelumbo nucifera Gaertn.spp. baijianlian)".JOURNAL OF PROTEOMICS 131(2016):61-70.