A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum

doi:10.3389/fpls.2016.01089

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	A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum
	Khaidun, A. B. M.2; Zhang, Chanjuan 3; Lv, Haiyan 2; Yuan, Ling 1; Wang, Ying 2; Huang, Wenjun 2; Chen, Jianjun 2
刊名	FRONTIERS IN PLANT SCIENCE
	2016-07-21
卷号	7 页码:12
关键词	Epimedium flavonoid flavonol MYB transcription factor
ISSN号	1664-462X
DOI	10.3389/fpls.2016.01089
英文摘要	Flavonols as plant secondary metabolites with vital roles in plant development and defense against UV light, have been demonstrated to be the main bioactive components (BCs) in the genus Epimedium plants, several species of which are used as materials for Herba Epimedii, an important traditional Chinese medicine. The flavonol biosynthetic pathway genes had been already isolated from Epimedium sagittatum, but a R2R3-MYB transcription factor regulating the flavonol synthesis has not been functionally characterized so far in Epimedium plants. In this study, we isolated and characterized the R2R3-MYB transcription factor EsMYBF1 involved in regulation of the flavonol biosynthetic pathway from E. sagittatum. Sequence analysis indicated that EsMYBF1 belongs to the subgroup 7 of R2R3-MYB family which contains the flavonol-specific MYB regulators identified to date. Transient reporter assay showed that EsMYBF1 strongly activated the promoters of EsF3H (flavanone 3-hydroxylase) and EsFLS (flavonol synthase), but not the promoters of EsDFRs (dihydroflavonol 4-reductase) and EsANS (anthocyanidin synthase) in transiently transformed Nicol-lane bentharniana leaves. Both yeast two-hybrid assay and transient reporter assay validated EsMYBF1 to be independent of EsTT8, or At) 18 bHLH regulators of the flavonoid pathway as cofactors. Ectopic expression of EsMYBF1 in transgenic tobacco resulted in the increased flavonol content and the decreased anthocyanin content in flowers. Correspondingly, the structural genes involved in flavonol synthesis were upregulated in the EsMYBF1 overexpression lines, including NtCHS (chalcone synthase), NtCHI (chalcone isomerase), NtF3H and NtFLS, whereas the late biosynthetic genes of the anthocyanin pathway (NtDFR and NtANS) were remarkably downregulated, compared to the controls. These results suggest that EsMYBF1 is a flavonol-specific R2R3-MYB regulator, and involved in regulation of the biosynthesis of the flavonol-derived BCs in E. sagittatum. Thus, identification and functional characterization of EsMYBF1 provide insight into understanding the biosynthesis and regulation of the flavonol-derived BCs in Epimedium plants, and also provide an effective tool gene for genetic manipulation to improve the flavonol synthesis.
资助项目	National Natural Science Foundation of China[31270340] ; National Natural Science Foundation of China[31200225]
WOS研究方向	Plant Sciences
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000380009200001
内容类型	期刊论文
源URL	[http://202.127.146.157/handle/2RYDP1HH/45]
专题	中国科学院武汉植物园
通讯作者	Wang, Ying
作者单位	1.Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY USA 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 3.Chinese Acad Agr Sci, Oil Crops Res Inst, Minist Agr, Key Lab Oil Crop Biol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Khaidun, A. B. M.,Zhang, Chanjuan,Lv, Haiyan,et al. A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum[J]. FRONTIERS IN PLANT SCIENCE,2016,7:12.
APA	Khaidun, A. B. M..,Zhang, Chanjuan.,Lv, Haiyan.,Yuan, Ling.,Wang, Ying.,...&Chen, Jianjun.(2016).A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum.FRONTIERS IN PLANT SCIENCE,7,12.
MLA	Khaidun, A. B. M.,et al."A R2R3-MYB Transcription Factor Regulates the Flavonol Biosynthetic Pathway in a Traditional Chinese Medicinal Plant, Epimedium sagittatum".FRONTIERS IN PLANT SCIENCE 7(2016):12.