UPLC-MS/MS analysis for antioxidant components of Lycii Fructus based on spectrum-effect relationship | |
Zhang XF(张弦飞)1,2; Chen J(陈娟)1; Yang JL(杨军丽)1; Shi YP(师彦平)1 | |
刊名 | Talanta |
2018 | |
期号 | 180页码:389-395 |
关键词 | Lycii Fructus Antioxidant Activity Spectrum-effect Relationship Uplc-ms/ms |
ISSN号 | 0039-9140 |
DOI | 10.1016/j.talanta.2017.12.078 |
英文摘要 | Lycii Fructus is widely cultivated in the Northwest China. It is well-known for its antiaging effect in traditional Chinese medicines (TCMs), but the effective components are not clear. In this work, the ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used to study the antioxidant components of Lycii Fructus through analyzing the spectrum-effect relationship, and the positive correlation components with antioxidant activity were partially identified. The extractums of Lycii Fructus were adsorbed with macroporous resin, and then eluted with water and 30%, 60%, 90% ethanol in turn. The extract fraction eluted with 60% ethanol was determined as the best, and was taken for subsequent experiments. With the above separation method, UPLC fingerprints of thirty batches of Lycii Fructus (from different areas) were obtained, and thirty common peaks were selected through similarity analysis (SA). Combined with the data of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, the spectrum-effect relationship was studied. The results showed that the main peaks with antioxidant activity were P14, P26, P8, and P21 for DPPH, and P26, P14, P21, and P19 for ABTS. Using the UPLC-MS/MS data, peaks P14, P19, P21, and P30 were respectively identified as chlorogenic acid, quercetin, kaempferol, and isorhamnetin, and then the results were confirmed through comparison with the standards and other references. Finally, their strong antioxidant activities were validated experimentally. |
学科主题 | 分析化学与药物化学 |
资助项目 | 药物化学成分研究组 |
语种 | 英语 |
WOS记录号 | WOS:000424717900051 |
资助机构 | the National Natural Science Foundation of China (Nos. 21575150 ; 21775153 ; 81673325) ; the Scholar Program of West Light Project |
内容类型 | 期刊论文 |
源URL | [http://ir.licp.ac.cn/handle/362003/23723] |
专题 | 兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室 |
通讯作者 | Yang JL(杨军丽); Shi YP(师彦平) |
作者单位 | 1.CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, PR China 2.University of Chinese Academy of Sciences, Beijing 100049, PR China |
推荐引用方式 GB/T 7714 | Zhang XF,Chen J,Yang JL,et al. UPLC-MS/MS analysis for antioxidant components of Lycii Fructus based on spectrum-effect relationship[J]. Talanta,2018(180):389-395. |
APA | Zhang XF,Chen J,Yang JL,&Shi YP.(2018).UPLC-MS/MS analysis for antioxidant components of Lycii Fructus based on spectrum-effect relationship.Talanta(180),389-395. |
MLA | Zhang XF,et al."UPLC-MS/MS analysis for antioxidant components of Lycii Fructus based on spectrum-effect relationship".Talanta .180(2018):389-395. |
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