Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 植物分子生物学研究组

	Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids
	Zhao, Dake; Shen, Yong; Shi, Yana; Shi, Xingqiao; Qiao, Qin; Zi, Shuhui; Zhao, Erqiang; Yu, Diqiu; Kennelly, Edward J.
刊名	PHYTOCHEMISTRY
	2018
卷号	152 期号:x 页码:113-124
关键词	Aconitum Carmichaelii (Ranunculaceae) Fuzi Transcriptome Biosynthesis Aconitine-type C-19-diterpenoid Alkaloids Acute Toxicity Traditional Chinese Medicine (Tcm)
ISSN号	0031-9422
英文摘要	Aconitum carmichaelii has long been used as a traditional Chinese medicine, and its processed lateral roots are known commonly as fuzi. Aconitine-type C-19-diterpenoid alkaloids accumulating in the lateral roots are some of the main toxicants of this species, yet their biosynthesis remains largely unresolved. As a first step towards understanding the biosynthesis of aconitine-type C-19-diterpenoid alkaloids, we performed de novo transcriptome assembly and analysis of rootstocks and leaf tissues of Aconitum carmichaelii by next-generation sequencing. A total of 525 unigene candidates were identified as involved in the formation of C-19-diterpenoid alkaloids, including those encoding enzymes in the early steps of diterpenoid alkaloids scaffold biosynthetic pathway, such as ent-copalyl diphosphate synthases, entkaurene synthases, kaurene oxidases, cyclases, and key aminotransferases. Furthermore, candidates responsible for decorating of diterpenoid alkaloid skeletons were discovered from transcriptome sequencing of fuzi, such as monooxygenases, methyltransferase, and BAHD acyltransferases. In addition, 645 differentially expressed genes encoding transcription factors potentially related to diterpenoid alkaloids accumulation underground were documented. Subsequent modular domain structure phylogenetics and differential expression analysis led to the identification of BAHD acyltransferases possibly involved in the formation of acetyl and benzoyl esters of diterpenoid alkaloids, associated with the acute toxicity of fuzi. The transcriptome data provide the foundation for future research into the molecular basis for aconitine-type C-19-diterpenoid alkaloids biosynthesis in A. carmichaelii. (C) 2018 Published by Elsevier Ltd.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
URL标识	查看原文
语种	英语
公开日期	2018-07-31
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/10971]
专题	西双版纳热带植物园_植物分子生物学研究组
推荐引用方式 GB/T 7714	Zhao, Dake,Shen, Yong,Shi, Yana,et al. Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids[J]. PHYTOCHEMISTRY,2018,152(x):113-124.
APA	Zhao, Dake.,Shen, Yong.,Shi, Yana.,Shi, Xingqiao.,Qiao, Qin.,...&Kennelly, Edward J..(2018).Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids.PHYTOCHEMISTRY,152(x),113-124.
MLA	Zhao, Dake,et al."Probing the transcriptome of Aconitum carmichaelii reveals the candidate genes associated with the biosynthesis of the toxic aconitine-type C-19-diterpenoid alkaloids".PHYTOCHEMISTRY 152.x(2018):113-124.