Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface

doi:10.7150/thno.23674

	Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface
	Deng, Cuijun 1; Zhu, Huiying ; Li, Jiayi 2; Feng, Chun 1; Yao, Qingqiang 2; Wang, Liming 2; Chang, Jiang ; Wu, Chengtie
刊名	THERANOSTICS
	2018
卷号	8 期号:7 页码:1940
关键词	3D-printing strontium osteoarthritis osteochondral defects cartilage regeneration
ISSN号	1838-7640
DOI	10.7150/thno.23674
英文摘要	The cartilage lesion resulting from osteoarthritis (OA) always extends into subchondral bone. It is of great importance for simultaneous regeneration of two tissues of cartilage and subchondral bone. 3D-printed Sr-5(PO4)(2)SiO4 (SPS) bioactive ceramic scaffolds may achieve the aim of regenerating both of cartilage and subchondral bone. We hypothesized that strontium (Sr) and silicon (Si) ions released from SPS scaffolds play a crucial role in osteochondral defect reconstruction. Methods: SPS bioactive ceramic scaffolds were fabricated by a 3D-printing method. The SEM and ICPAES were used to investigate the physicochemical properties of SPS scaffolds. The proliferation and maturation of rabbit chondrocytes stimulated by SPS bioactive ceramics were measured in vitro. The stimulatory effect of SPS scaffolds for cartilage and subchondral bone regeneration was investigated in vivo. Results: SPS scaffolds significantly stimulated chondrocyte proliferation, and SPS extracts distinctly enhanced the maturation of chondrocytes and preserved chondrocytes from OA. SPS scaffolds markedly promoted the regeneration of osteochondral defects. The complex interface microstructure between cartilage and subchondral bone was obviously reconstructed. The underlying mechanism may be related to Sr and Si ions stimulating cartilage regeneration by activating HIF pathway and promoting subchondral bone reconstruction through activating Wnt pathway, as well as preserving chondrocytes from OA via inducing autophagy and inhibiting hedgehog pathway. Conclusion: Our findings suggest that SPS scaffolds can help osteochondral defect reconstruction and well reconstruct the complex interface between cartilage and subchondral bone, which represents a promising strategy for osteochondral defect regeneration.
学科主题	Medicine, Research & Experimental
出版者	IVYSPRING INT PUBL
WOS记录号	WOS:000425394000013
资助机构	This work was supported by the National Key Research and Development Program of China (2016YFB0700803), the Natural Science Foundation of China (51761135103, 81771989, 81601612), Key Research Program of Frontier Sciences CAS (QYZDB-SSW-SYS027), Science and Technology Commission of Shanghai Municipality (17441903700, 16DZ2260603) and Key Research Program of Science & Technology Support Program of Jiangsu Province (BE2016763). ; This work was supported by the National Key Research and Development Program of China (2016YFB0700803), the Natural Science Foundation of China (51761135103, 81771989, 81601612), Key Research Program of Frontier Sciences CAS (QYZDB-SSW-SYS027), Science and Technology Commission of Shanghai Municipality (17441903700, 16DZ2260603) and Key Research Program of Science & Technology Support Program of Jiangsu Province (BE2016763).
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/24589]
专题	中国科学院上海硅酸盐研究所
作者单位	1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanjing Med Univ, Digital Med Inst, Dept Orthopaed Surg, Nanjing Hosp, 68 Changle Rd, Nanjing 210006, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Deng, Cuijun,Zhu, Huiying,Li, Jiayi,et al. Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface[J]. THERANOSTICS,2018,8(7):1940, 1955.
APA	Deng, Cuijun.,Zhu, Huiying.,Li, Jiayi.,Feng, Chun.,Yao, Qingqiang.,...&Wu, Chengtie.(2018).Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface.THERANOSTICS,8(7),1940.
MLA	Deng, Cuijun,et al."Bioactive Scaffolds for Regeneration of Cartilage and Subchondral Bone Interface".THERANOSTICS 8.7(2018):1940.