OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP

doi:10.1111/jpy.12623

	OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP
	Wei, Li 1; Xu, Jian 1
刊名	JOURNAL OF PHYCOLOGY
	2018-06-01
卷号	54 期号:3 页码:358-367
关键词	ChIP-qPCR ChIP-Seq chromatin immunoprecipitation epigenomics histone modifications Nannochloropsis oceanica
ISSN号	0022-3646
DOI	10.1111/jpy.12623
英文摘要	Epigenetic factors such as histone modifications play integral roles in plant development and stress response, yet their implications in algae remainpoorly understood. In the industrial oleaginousmicroalgae Nannochloropsis spp., the lack of an efficient methodology for chromatin immunoprecipitation (ChIP), which determines the specific genomic location of various histone modifications, has hindered probing the epigenetic basis of their photosynthetic carbon conversion and storage as oil. Here, a detailed ChIP protocol was developed for Nannochloropsis oceanica, which represents a reliable approach for the analysis of histone modifications, chromatin state, and transcription factor-binding sites at the epigenetic level. Using ChIP-qPCR, genes related to photosynthetic carbon fixation in this microalga were systematically assessed. Furthermore, a ChIP-Seq protocol was established and optimized, which generated a genome-wide profile of histone modification events, using histone mark H3K9Ac as an example. These results are the first step for appreciation of the chromatin landscape in industrial oleaginous microalgae and for epigenetics-based microalgal feedstock development.
资助项目	National Natural Science Foundation of China[31741005] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[ZDRW-ZS-2016-3] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[KSZD-EW-Z-017]
WOS关键词	OLEAGINOUS MICROALGAE ; LIPID PRODUCTION ; TRIACYLGLYCEROL BIOSYNTHESIS ; ALGA NANNOCHLOROPSIS ; OCEANICA CCMP1779 ; GADITANA ; MODEL ; ACYLTRANSFERASES ; OVEREXPRESSION ; MECHANISMS
WOS研究方向	Plant Sciences ; Marine & Freshwater Biology
语种	英语
出版者	WILEY
WOS记录号	WOS:000435798700005
资助机构	National Natural Science Foundation of China ; Biological Carbon Sequestration Program from Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/12507]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wei, Li; Xu, Jian
作者单位	1.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wei, Li,Xu, Jian. OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP[J]. JOURNAL OF PHYCOLOGY,2018,54(3):358-367.
APA	Wei, Li,&Xu, Jian.(2018).OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP.JOURNAL OF PHYCOLOGY,54(3),358-367.
MLA	Wei, Li,et al."OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP".JOURNAL OF PHYCOLOGY 54.3(2018):358-367.