Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)

doi:10.1111/anu.12688

	Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)
	Liu, Xinwei 1,2,3; Wang, Mengqiang 1,2; Sun, Guoqiong 1,2; Wang, Baojie 1,2; Jiang, Keyong 1,2; Shao, Jianchun 1,2,3; Qi, Cancan 1,2,3; Zhao, Wei 4; Han, Siyin 1,2,3; Liu, Mei 1,2
刊名	AQUACULTURE NUTRITION
	2018-10-01
卷号	24 期号:5 页码:1509-1520
关键词	autophagy Litopenaeus vannamei mTOR rapamycin transcriptome
ISSN号	1353-5773
DOI	10.1111/anu.12688
英文摘要	Mechanistic target of rapamycin (mTOR) is a central regulator of cell growth and metabolism including autophagy. The mTORC1 signalling pathway is a major regulator of autophagy in response to nutrient levels. The drug rapamycin induces autophagy in various cell types and species by specifically inhibiting the activity of mTOR. To identify the genes and correlative regulatory pathways involved in cell metabolism and autophagy of Litopenaeus vannamei, we analysed the transcriptome of shrimps treated with rapamycin. We found that the expression of some genes related to autophagy was significantly changed. To further explore the autophagy regulatory mechanism, the concentration and phosphorylation of related proteins were analysed by Western blot. We found that treatment with the rapamycin can depress phosphorylating of ATG1 and promote dephosphorylating of ATG13. There are some heat-shock protein genes and genes involving apoptosis, function of lysosome and feedback regulation of mTOR signalling path differently expressed. The relationship between them and autophagy induced by inhibiting mTOR is also discussed. Evidences show the complicated positive and negative feedback loops controlling autophagy crustaceans are scarce. Our study verifies and contributes to the current understanding of autophagy induced by mTOR in crustacean, providing an abundant source for the identification of novel genes.
资助项目	National Natural Science Foundation of China[41406151] ; Science and Technology Major Project of Shandong Province[2015ZDZX05002] ; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106]
WOS关键词	HEAT-SHOCK-RESPONSE ; CELL-GROWTH ; EMERGING ROLE ; S6 KINASE ; IN-VIVO ; PROTEIN ; MTOR ; TOR ; PHOSPHORYLATION ; APOPTOSIS
WOS研究方向	Fisheries
语种	英语
出版者	WILEY
WOS记录号	WOS:000443545000014
资助机构	National Natural Science Foundation of China ; Science and Technology Major Project of Shandong Province ; Chinese Academy of Sciences STS Major Deployment Project
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11884]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Liu, Mei; Wang, Lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Tianjin Agr Univ, Coll Fishery Sci, Tianjin Key Lab Aquaculture, Tianjin, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xinwei,Wang, Mengqiang,Sun, Guoqiong,et al. Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)[J]. AQUACULTURE NUTRITION,2018,24(5):1509-1520.
APA	Liu, Xinwei.,Wang, Mengqiang.,Sun, Guoqiong.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2018).Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei).AQUACULTURE NUTRITION,24(5),1509-1520.
MLA	Liu, Xinwei,et al."Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)".AQUACULTURE NUTRITION 24.5(2018):1509-1520.