Recent advances in the selection and identification of antigen-specific nanobodies

doi:10.1016/j.molimm.2018.02.012

	Recent advances in the selection and identification of antigen-specific nanobodies
	Liu, Wenshuai 1; Song, Haipeng 2; Chen, Quan 1; Yu, Jianli 2; Xian, Mo 1; Nian, Rui 1; Feng, Dongxiao 3
刊名	MOLECULAR IMMUNOLOGY
	2018-04-01
卷号	96 页码:37-47
关键词	Nanobody Vhh Antigen-specific Display Technology Library
ISSN号	0161-5890
DOI	10.1016/j.molimm.2018.02.012
文献子类	Review
英文摘要	Nanobodies represent the next-generation antibody-derived biologics with significant advances over conventional antibodies. Several rapid and robust techniques for isolating highly specific nanobodies have been developed. Antigen specific nanobodies are selected from constructed nanobody libraries, which can be classified into 3 main types: immune library, naive library, and semisynthetic/synthetic library. The immune library is the most widely used strategy for nanobody screening. Target specific nanobodies are highly enriched in immune libraries than in non-immune libraries; however, it is largely limited by the natural antigenicity of antigens. The naive library is thus developed. Despite the lack of somatic maturation, protein engineering can be employed to significantly increase the affinities of selected binders. However, a substantial amount of blood samples collected from a large number of individual animals is a prerequisite to ensure the diversity of the naive library. With this issue considered, the semisynthetic/synthetic library may be a promising path toward obtaining a limitless source of nanobodies against a variety of antigens without the need of animals. In this review, we summarize the state-of-the-art screening technologies with different libraries. The approaches presented here can further boost the diverse applications of nanobodies in biomedicine and biotechnology.
WOS关键词	SINGLE-DOMAIN ANTIBODY ; HEAVY-CHAIN ANTIBODIES ; IN-VITRO ; RIBOSOME-DISPLAY ; AFFINITY MATURATION ; PHAGE DISPLAY ; FUNCTIONAL PROTEINS ; SURFACE DISPLAY ; RAPID ISOLATION ; MICROCYSTIN-LR
WOS研究方向	Biochemistry & Molecular Biology ; Immunology
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000430647700005
资助机构	Nanobodi-QIBEBT Collaboration Project(20163702021237) ; National Natural Science Foundation of China(21676286 ; Primary Research Development Plan of Shandong Province(2016GSF121006) ; 81702085)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/11509]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Nian, Rui; Feng, Dongxiao
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Shenzhen Innova Nanobodi Co Ltd, 7018 Caitian Rd, Shenzhen 518000, Peoples R China 3.Binzhou Med Univ, Sch Pharmaceut Sci, 346 Guanhai Rd, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenshuai,Song, Haipeng,Chen, Quan,et al. Recent advances in the selection and identification of antigen-specific nanobodies[J]. MOLECULAR IMMUNOLOGY,2018,96:37-47.
APA	Liu, Wenshuai.,Song, Haipeng.,Chen, Quan.,Yu, Jianli.,Xian, Mo.,...&Feng, Dongxiao.(2018).Recent advances in the selection and identification of antigen-specific nanobodies.MOLECULAR IMMUNOLOGY,96,37-47.
MLA	Liu, Wenshuai,et al."Recent advances in the selection and identification of antigen-specific nanobodies".MOLECULAR IMMUNOLOGY 96(2018):37-47.