Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli

doi:10.1186/s13068-018-1297-3

	Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli
	Liu, Changqing 1,2; Zhang, Kai 1,2; Cao, Wenyan 1,2; Zhang, Ge 1,2,3; Chen, Guoqiang 1,2,3; Yang, Haiyan 1,2; Wang, Qian 3; Liu, Haobao 3; Xian, Mo 1,2; Zhang, Haibo 1,2
刊名	BIOTECHNOLOGY FOR BIOFUELS
	2018-11-08
卷号	11 页码:15
关键词	2-Phenylethanol Enterobacter sp CGMCC 5087 2-Keto acid decarboxylase Alcohol dehydrogenase Phenylpyruvate pathway Metabolic engineering
ISSN号	1754-6834
DOI	10.1186/s13068-018-1297-3
英文摘要	Background2-Phenylethanol (2-PE) is a higher aromatic alcohol that is widely used in the perfumery, cosmetics, and food industries and is also a potentially valuable next-generation biofuel. In our previous study, a new strain Enterobacter sp. CGMCC 5087 was isolated to produce 2-PE from glucose through the phenylpyruvate pathway.ResultsIn this study, candidate genes for 2-PE biosynthesis were identified from Enterobacter sp. CGMCC 5087 by draft whole-genome sequence, metabolic engineering, and shake flask fermentation. Subsequently, the identified genes encoding the 2-keto acid decarboxylase (Kdc) and alcohol dehydrogenase (Adh) enzymes from Enterobacter sp. CGMCC 5087 were introduced into E. coli BL21(DE3) to construct a high-efficiency microbial cell factory for 2-PE production using the prokaryotic phenylpyruvate pathway. The enzymes Kdc4427 and Adh4428 from Enterobacter sp. CGMCC 5087 showed higher performances than did the corresponding enzymes ARO10 and ADH2 from Saccharomyces cerevisiae, respectively. The E. coli cell factory was further improved by overexpressing two upstream shikimate pathway genes, aroF/aroG/aroH and pheA, to enhance the metabolic flux of the phenylpyruvate pathway, which resulted in 2-PE production of 260mg/L. The combined overexpression of tktA and ppsA increased the precursor supply of erythrose-4-phosphate and phosphoenolpyruvate, which resulted in 2-PE production of 320mg/L, with a productivity of 13.3mg/L/h.ConclusionsThe present study achieved the highest titer of de novo 2-PE production of in a recombinant E. coli system. This study describes a new, efficient 2-PE producer that lays foundation for the industrial-scale production of 2-PE and its derivatives in the future.
资助项目	National Natural Science Foundation of China (NSF)[31400084] ; Hainan's Key Project of Research and Development Plan[ZDYF2017155] ; Taishan Scholars Climbing Program of Shandong[TSPD20150210] ; Youth Innovation Promotion Association CAS[2017252]
WOS关键词	SACCHAROMYCES-CEREVISIAE ; EHRLICH PATHWAY ; L-PHENYLALANINE ; PHENYLPYRUVATE DECARBOXYLASE ; PHENYLACETALDEHYDE SYNTHASE ; BIOTECHNOLOGICAL PRODUCTION ; METABOLISM ; BIOFUELS ; YEAST ; BIOCATALYSTS
WOS研究方向	Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	BMC
WOS记录号	WOS:000449850300004
资助机构	National Natural Science Foundation of China (NSF) ; Hainan's Key Project of Research and Development Plan ; Taishan Scholars Climbing Program of Shandong ; Youth Innovation Promotion Association CAS
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10998]
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Xian, Mo; Zhang, Haibo
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Agr Sci, Key Lab Tobacco Gene Resources, Tobacco Res Inst, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Changqing,Zhang, Kai,Cao, Wenyan,et al. Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11:15.
APA	Liu, Changqing.,Zhang, Kai.,Cao, Wenyan.,Zhang, Ge.,Chen, Guoqiang.,...&Zhang, Haibo.(2018).Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli.BIOTECHNOLOGY FOR BIOFUELS,11,15.
MLA	Liu, Changqing,et al."Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli".BIOTECHNOLOGY FOR BIOFUELS 11(2018):15.