Lipase@ZIF-8 nanoparticles-based biosensor for direct and sensitive detection of methyl parathion

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2018专题

	Lipase@ZIF-8 nanoparticles-based biosensor for direct and sensitive detection of methyl parathion
	Tan, Chin-Ping; Weng, Xinchu; Cheong, Ling-Zhi; Ma, Baokai; Shen, Cai
刊名	ELECTROCHIMICA ACTA
	2018
卷号	283 页码:509-516
关键词	Zeolitic Imidazolate Frameworks Metal-organic Frameworks Organophosphate Pesticides Biomimetic Mineralization Rapid Detection Water Extraction Efficient Hydrolase Samples
英文摘要	Organophosphorus pesticides especially p-nitrophenyl pesticides (methyl parathion) have neurotoxic effects and it is a difficult to remove organophosphorus pesticides residues from contaminated water. Present work has developed a sensitive, direct and rapid lipase@ZIF-8/chitosan/glassy carbon electrode (GCE) and lipase@amine-modified ZIF-8(An-ZIF-8)/chitosan/GCE biosensors for detection of methyl parathion. ZIF-8 and An-ZIF-8 nanoparticles which are a type of metal-organic frameworks with large surface area and high porosity have been facilely prepared under mild biocompatible conditions and used as lipase immobilization matrixes in the biosensors. The optimized lipase@ZIF-8/chitosan/GCE and lipase@An-ZIF-8/chitosan/GCE biosensors can detect methyl parathion with a wide linear range, good repeatability, recovery and limit of detection as low as 0.28 mu M. The as-prepared biosensors can be stored in 0.05 M PBS (pH 7) at 4 degrees C and reused for at least 20 days with a retainment of at least 80% of its initial response to methyl parathion. This indicated that both ZIF-8/chitosan and An-ZIF-8/chitosan are suitable for immobilization of lipase that favors generation of p-nitrophenol from methyl parathion; and subsequent electrochemical oxidation and electron transfer of the p-nitrophenol. (C) 2018 Elsevier Ltd. All rights reserved.
学科主题	Physics
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/17227]
专题	2018专题
推荐引用方式 GB/T 7714	Tan, Chin-Ping,Weng, Xinchu,Cheong, Ling-Zhi,et al. Lipase@ZIF-8 nanoparticles-based biosensor for direct and sensitive detection of methyl parathion[J]. ELECTROCHIMICA ACTA,2018,283:509-516.
APA	Tan, Chin-Ping,Weng, Xinchu,Cheong, Ling-Zhi,Ma, Baokai,&Shen, Cai.(2018).Lipase@ZIF-8 nanoparticles-based biosensor for direct and sensitive detection of methyl parathion.ELECTROCHIMICA ACTA,283,509-516.
MLA	Tan, Chin-Ping,et al."Lipase@ZIF-8 nanoparticles-based biosensor for direct and sensitive detection of methyl parathion".ELECTROCHIMICA ACTA 283(2018):509-516.