Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2018专题

	Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies
	Mariuba, L. A.; Chaves, Y. O.; Barbosa, L.; Iqbal, M. Zubair; Faria, F. S. E. D. V.; Morales, M. A.; dos Santos, C. C.; Rocha, C. O.; Piazza, R. D.; Rodriguez, A. F. R.
刊名	NANOTECHNOLOGY
	2018
卷号	29 期号:36
关键词	Iron-oxide Nanoparticles Magnetic Nanoparticles Flow-cytometry Bioseparations
英文摘要	Magnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (gamma Fe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-gamma Fe2O3 beads were converted to carboxylic acid (CARB-gamma Fe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-gamma Fe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mossbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.
学科主题	Materials Science
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/17209]
专题	2018专题
推荐引用方式 GB/T 7714	Mariuba, L. A.,Chaves, Y. O.,Barbosa, L.,et al. Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies[J]. NANOTECHNOLOGY,2018,29(36).
APA	Mariuba, L. A..,Chaves, Y. O..,Barbosa, L..,Iqbal, M. Zubair.,Faria, F. S. E. D. V..,...&Jafelicci, M., Jr..(2018).Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies.NANOTECHNOLOGY,29(36).
MLA	Mariuba, L. A.,et al."Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies".NANOTECHNOLOGY 29.36(2018).