OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice

CORC > 宁波材料技术与工程研究所 > 中国科学院宁波材料技术与工程研究所 > 2018专题

	OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice
	Zheng, W. J.; Wang, S. S.; Zhu, X. N.; Lin, J. X.; Zhang, B. T.; Zhou, J. Q.; Ni, J.; Pan, Z. C.; Zhu, S. H.; Ding, W. N.
刊名	BIOLOGIA PLANTARUM
	2018
卷号	62 期号:4 页码:732-740
关键词	Plant-cell Growth Oryza-sativa Genetic-control Arabidopsis Morphogenesis Grasses Pattern Stress Plays L.
英文摘要	Root hairs play important roles in plant nutrient and water acquisition. To better understand the genetic mechanism controlling root hair development in rice (Oryza sativa L.), a rice mutant with root hair defects was isolated and characterized. Cryo-scanning electron microscope showed that the density and length of root hairs in the mutant were significantly reduced compared to the wild type (WT). Map-based cloning and complementation test revealed that the mutation occurred in a NADPH oxidase gene OsNOX3 (LOC_Os01g61880). The OsNOX3 displays high sequence similarity with the previously characterized NOX genes RTH5 in maize and RHD2 in Arabidopsis, which play critical roles in root hair development. Expression pattern analysis indicated that OsNOX3 was expressed in various tissues throughout the plant with high expression in roots and root hairs. Subcellular localization analysis confirmed that OsNOX3 was located on the plasma membrane. Staining assays showed that the content of superoxide and hydrogen peroxide were significantly reduced in root hair tips of Osnox3 when compared to WT. Our results showed critical roles of OsNOX3 in regulating both root hair initiation and elongation in rice, which is similar to RTH5 but different from RHD2, confirming the difference of genetic mechanisms regulating root hair morphogenesis in monocot and dicot plants.
学科主题	Materials Science
语种	英语
公开日期	2018-12-04
内容类型	期刊论文
源URL	[http://ir.nimte.ac.cn/handle/174433/16989]
专题	2018专题
推荐引用方式 GB/T 7714	Zheng, W. J.,Wang, S. S.,Zhu, X. N.,et al. OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice[J]. BIOLOGIA PLANTARUM,2018,62(4):732-740.
APA	Zheng, W. J..,Wang, S. S..,Zhu, X. N..,Lin, J. X..,Zhang, B. T..,...&Ding, W. N..(2018).OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice.BIOLOGIA PLANTARUM,62(4),732-740.
MLA	Zheng, W. J.,et al."OsNOX3, encoding a NADPH oxidase, regulates root hair initiation and elongation in rice".BIOLOGIA PLANTARUM 62.4(2018):732-740.