Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs

doi:10.1039/c8tb00376a

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	Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs
	Zhang, Jiabin 1; Yun, Seonho 1; Bi, Jingxiu 1; Dai, Sheng 1; Du, Yuguang 2; Zannettino, Andrew C. W.3; Zhang, Hu 1
刊名	JOURNAL OF MATERIALS CHEMISTRY B
	2018-03-28
卷号	6 期号:12 页码:1799-1814
ISSN号	2050-750X
DOI	10.1039/c8tb00376a
文献子类	Article
英文摘要	Human mesenchymal stem/stromal cells (hMSCs) are a potential cell source of stem cell therapy for many serious diseases and hMSC spheroids have emerged to replace single cell suspensions for cell therapy. Three-dimensional (3D) scaffolds or hydrogels which can mimic properties of the extracellular matrix (ECM) have been widely explored for their application in tissue regeneration. However, there are considerably less studies on inducing differentiation of hMSC spheroids using 3D scaffolds or hydrogels. This study is the first to explore multi-lineage differentiation of a stem cell line and primary stem cells within poly(N-isopropylacrylamide) (p(NIPAAm))-based thermosensitive microgel-formed constructs. We first demonstrated that poly(N-isopropylacrylamide-co-acrylic acid) (p(NIPAAm-AA)) was not toxic to hMSCs and the microgel-formed constructs facilitated formation of uniform stem cell spheroids. Due to functional enhancement of cell spheroids, hMSCs within the 3D microgel-formed constructs were induced for multi-lineage differentiation as evidenced by significant up-regulation of messenger RNA (mRNA) expression of chondrogenic and osteogenic genes even in the absence of induction media on day 9. When induction media were in situ supplied on day 9, mRNA expression of chondrogenic, osteogenic and adipogenic genes within the microgel-formed constructs were significantly higher than that in the pellet and 2D cultures, respectively, on day 37. In addition, histological and immunofluorescent images also confirmed successful multi-lineage differentiation of hMSCs within the 3D microgel-formed constructs. Hence, the thermosensitive p(NIPAAm-AA) microgel can be potentially used in an in vitro model for cell differentiation or in vivo transplantation of pre-differentiated human mesenchymal stromal cells into patients for specific lineage differentiation.
WOS关键词	INTERVERTEBRAL DISC REGENERATION ; STEM-CELLS ; PRECURSOR CELLS ; STROMAL CELLS ; OSTEOGENIC DIFFERENTIATION ; PENTOSAN POLYSULFATE ; INJECTABLE HYDROGEL ; OVINE MODEL ; REPAIR ; CULTURES
WOS研究方向	Materials Science
语种	英语
WOS记录号	WOS:000428986900009
资助机构	IPE-UoA Scholarship (BES) ; ARC Discovery Project(DP160104632) ; Medical Advancement Without Animal (MAWA) Trust
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/24177]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Univ Adelaide, Sch Chem Engn, Adelaide, SA 5005, Australia 2.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 3.Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5001, Australia
推荐引用方式 GB/T 7714	Zhang, Jiabin,Yun, Seonho,Bi, Jingxiu,et al. Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs[J]. JOURNAL OF MATERIALS CHEMISTRY B,2018,6(12):1799-1814.
APA	Zhang, Jiabin.,Yun, Seonho.,Bi, Jingxiu.,Dai, Sheng.,Du, Yuguang.,...&Zhang, Hu.(2018).Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs.JOURNAL OF MATERIALS CHEMISTRY B,6(12),1799-1814.
MLA	Zhang, Jiabin,et al."Enhanced multi-lineage differentiation of human mesenchymal stem/stromal cells within poly(N-isopropylacrylamide-acrylic acid) microgel-formed three-dimensional constructs".JOURNAL OF MATERIALS CHEMISTRY B 6.12(2018):1799-1814.