A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors | |
Mao, Rui1,2,3; Ge, Ge1,2; Wang, Zhuo1,2; Hao, Rongzhang5; Zhang, Guohao1,2; Yang, Zhenzhou1,2; Lin, Bincheng1,2; Ma, Yajun4; Liu, Hongtao1,2; Du, Yuguang1,2 | |
刊名 | ACTA TROPICA |
2018-02-01 | |
卷号 | 178页码:86-92 |
关键词 | Anopheles Mosquito Plasmodium Loop-mediated Isothermal Amplification (Lamp) Multiplex Microfluidic Chip Morphological Analysis |
ISSN号 | 0001-706X |
DOI | 10.1016/j.actatropica.2017.10.025 |
文献子类 | Article |
英文摘要 | Malaria infection poses a great threaten to public health even nowadays. The conventional diagnosis tools of malaria parasites and vectors require systematic training for the observers accompanied by the low throughput. In this study, a new detection system, i.e., multiplex microfluidic loop-mediated isothermal amplification (m mu LAMP) array, was developed to provide a convenient, rapid and economical detection system for malaria diagnosis. A microfluidic-based detection chip was designed and developed, targeting the conserved gene of four Anopheles and two Plasmodium species responsible for most of the malaria cases occurred in China. The DNA preparation of Anopheles and Plasmodium samples was realized by using a newly-developed DNA extraction method. For this m mu LAMP array system, the detection limit was determined to be 1 pg of targeting DNA with high sensitivity (>95%) and specificity (100%). Further, the accuracy of such m mu LAMP analysis was evaluated by the analysis of 48 Anopheles mosquito samples, of which 30 were termed to be target Anopheles, displaying high consistency with that by morphological analysis. In conclusion, the m mu LAMP detection system was proved to be a visible, sensitive, specific and high-throughput diagnostic tool. Considering the portable manipulation of such detection system, our studies shed light on its potential application of malaria surveillance on the spot. |
WOS关键词 | Rapid Identification ; Lamp ; Diagnosis ; Mosquitos ; Microscopy ; Devices |
WOS研究方向 | Parasitology ; Tropical Medicine |
语种 | 英语 |
WOS记录号 | WOS:000423644300013 |
资助机构 | Chinese academy of sciences(CXJJ-15Z012) ; National High Technology Research and Development Program of China(2015AA020929) ; Beijing Nova Program(Z141107001814071) |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/23987] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Key Lab Biopharmaceut Prod & Formulat Engn, PLA, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Second Mil Med Univ, Dept Pathogen Biol, Shanghai 200433, Peoples R China 5.Acad Mil Med Sci, Inst Dis Control & Prevent, Beijing 100071, Peoples R China |
推荐引用方式 GB/T 7714 | Mao, Rui,Ge, Ge,Wang, Zhuo,et al. A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors[J]. ACTA TROPICA,2018,178:86-92. |
APA | Mao, Rui.,Ge, Ge.,Wang, Zhuo.,Hao, Rongzhang.,Zhang, Guohao.,...&Du, Yuguang.(2018).A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors.ACTA TROPICA,178,86-92. |
MLA | Mao, Rui,et al."A multiplex microfluidic loop-mediated isothermal amplification array for detection of malaria-related parasites and vectors".ACTA TROPICA 178(2018):86-92. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论