Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification | |
Khan, M. Kamran1,2; Luo, Jianquan1,2; Khan, Rashid1,2; Fan, Jinxin1,2; Wan, Yinhua1,2 | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2017-10-27 | |
卷号 | 1521页码:19-26 |
关键词 | Membrane Chromatography Alginate Dialdehyde Lysozyme Protein Purification Microfiltration |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2017.09.031 |
文献子类 | Article |
英文摘要 | Fabricating membrane adsorbers with high adsorption capacity and appreciable throughput for the separation and purification of protein products is challenging in biomedical and pharmaceutical industries. Herein, we report the synthesis of a novel membrane adsorber by functionalizing a nylon microfiltration membrane with alginate dialdehyde (ADA) followed by sulphonic addition, without any solvent usage, and its successful application in the purification of lysozyme. Taking advantage of abundant dual cation exchange (CEX) groups on sulphonic-ADA (S-ADA) ligands, this novel S-ADA-nylon membrane adsorber showed an unprecedented static binding capicity of 286 mg/mL for lysozyme adsorption. Meanwhile, the prepared membrane adsorber could be easily regenerated (complete protein elution) under mild conditions and be reused at least for five times. Featured with a unique selectivity, the S-ADA-nylon membrane also captured lysozyme from chicken egg white solution with a high purity (100%) and a high recovery of 98%. The purified lysozyme showed similar specific activity as commercial product. The present work provides a facile, green and low-cost approach for the preparation of high-performance membrane adsorbers, which has a great potential in protein production. (C) 2017 Elsevier B.V. All rights reserved. |
WOS关键词 | Monoclonal-antibody ; Step-purification ; Chromatography ; Separation ; Lysozyme ; Clearance ; Cryogel ; Design ; Virus ; Beads |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000413382800003 |
资助机构 | National Science Foundation of China(21506229) ; Youth Innovation Promotion Association of Chinese Academy of Sciences(2017069) ; '100 Talents Program' of Chinese Academy of Sciences |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/23411] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Inst Proc Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,et al. Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1521:19-26. |
APA | Khan, M. Kamran,Luo, Jianquan,Khan, Rashid,Fan, Jinxin,&Wan, Yinhua.(2017).Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification.JOURNAL OF CHROMATOGRAPHY A,1521,19-26. |
MLA | Khan, M. Kamran,et al."Facile and green fabrication of cation exchange membrane adsorber with unprecedented adsorption capacity for protein purification".JOURNAL OF CHROMATOGRAPHY A 1521(2017):19-26. |
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