Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio)

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	Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio)
	Xu, Jing; Huang, Wei; Zhong, Chengrong; Luo, Daji; Li, Shuangfei; Zhu, Zuoyan; Hu, Wei; Xu, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; huwei@ihb.ac.cn
刊名	PLOS ONE
	2011
卷号	6 期号:6 页码:e21057
关键词	Gonadotropin-releasing-hormone Suppression Subtractive Hybridization Complementary-dna Subtraction Goldfish Carassius-auratus C-type Lectin Microarray Analysis Fish Gonadotropins Ovarian-function Growth-hormone 3 Forms
ISSN号	1932-6203
英文摘要	Background: The hypothalamic-pituitary-gonadal (HPG) axis is critical in the development and regulation of reproduction in fish. The inhibition of neuropeptide gonadotropin-releasing hormone (GnRH) expression may diminish or severely hamper gonadal development due to it being the key regulator of the axis, and then provide a model for the comprehensive study of the expression patterns of genes with respect to the fish reproductive system. Methodology/Principal Findings: In a previous study we injected 342 fertilized eggs from the common carp (Cyprinus carpio) with a gene construct that expressed antisense sGnRH. Four years later, we found a total of 38 transgenic fish with abnormal or missing gonads. From this group we selected the 12 sterile females with abnormal ovaries in which we combined suppression subtractive hybridization (SSH) and cDNA microarray analysis to define changes in gene expression of the HPG axis in the present study. As a result, nine, 28, and 212 genes were separately identified as being differentially expressed in hypothalamus, pituitary, and ovary, of which 87 genes were novel. The number of down-and up-regulated genes was five and four (hypothalamus), 16 and 12 (pituitary), 119 and 93 (ovary), respectively. Functional analyses showed that these genes involved in several biological processes, such as biosynthesis, organogenesis, metabolism pathways, immune systems, transport links, and apoptosis. Within these categories, significant genes for neuropeptides, gonadotropins, metabolic, oogenesis and inflammatory factors were identified. Conclusions/Significance: This study indicated the progressive scaling-up effect of hypothalamic sGnRH antisense on the pituitary and ovary receptors of female carp and provided comprehensive data with respect to global changes in gene expression throughout the HPG signaling pathway, contributing towards improving our understanding of the molecular mechanisms and regulative pathways in the reproductive system of teleost fish.
学科主题	Life Sciences & Biomedicine - Other Topics
语种	英语
WOS记录号	WOS:000291612600044
资助机构	Development Plan of the state Key Fundamental Research of China[2007CB109206]; National Natural Science Foundation[30930069]; Hubei Provincial Foundation for Distinguished Youth Scholar[2008ABB010] ; Development Plan of the state Key Fundamental Research of China[2007CB109206]; National Natural Science Foundation[30930069]; Hubei Provincial Foundation for Distinguished Youth Scholar[2008ABB010] ; Development Plan of the state Key Fundamental Research of China[2007CB109206]; National Natural Science Foundation[30930069]; Hubei Provincial Foundation for Distinguished Youth Scholar[2008ABB010] ; Development Plan of the state Key Fundamental Research of China[2007CB109206]; National Natural Science Foundation[30930069]; Hubei Provincial Foundation for Distinguished Youth Scholar[2008ABB010]
公开日期	2011-12-22
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16447]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者	Xu, J (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China; huwei@ihb.ac.cn
推荐引用方式 GB/T 7714	Xu, Jing,Huang, Wei,Zhong, Chengrong,et al. Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio)[J]. PLOS ONE,2011,6(6):e21057.
APA	Xu, Jing.,Huang, Wei.,Zhong, Chengrong.,Luo, Daji.,Li, Shuangfei.,...&huwei@ihb.ac.cn.(2011).Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio).PLOS ONE,6(6),e21057.
MLA	Xu, Jing,et al."Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio)".PLOS ONE 6.6(2011):e21057.