Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)

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	Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)
	Li, Jie; Ou, Danyun; Zheng, Lingling; Gan, Nanqin; Song, Lirong; Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; lrsong@ihb.ac.cn
刊名	PHYCOLOGICAL RESEARCH
	2011
卷号	59 期号:3 页码:200-207
关键词	Esterase Activity Fluorescein Diacetate Low Temperature Metabolic Activity Metabolic Vigor Photosynthetic Activity
ISSN号	1322-0829
英文摘要	The fluorescein diacetate (FDA) assay has been widely used to measure metabolic activity in phytoplankton. It was found that FDA fluorescence values did not decrease in some stressed cells, demonstrating that the applicability of the method needs to be assessed further in the context of growth-influencing conditions. In the present study, changes of FDA fluorescence values were studied in bloom-forming cyanobacterial Microcystis aeruginosa Kutz cells under stress conditions such as nitrogen (N) or phosphorus (P) deficiency, or darkness and low temperature (10 degrees C), respectively. The results demonstrated that esterase activity decreased immediately in dark-stressed cells, which correlated with the decline of biomass and photosynthetic activity. Under the other three stress conditions, however, especially at low temperature, the cells lost photosynthetic activity but had the highest esterase activity, which was five times higher than the control group. These findings contrast with the assay criteria that the expression of a stain should reflect the change of photosynthetic activity and that stressed cells should have a lower staining intensity than the control cells. According to these results, the esterase activity response was dependent on environmental factors. Furthermore, higher fluorescence intensity did not mean higher metabolic activity, but a discrepant value indicated a severe stress.
学科主题	Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000292157900008
资助机构	National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1] ; National Basic Research Program of China[2008CB418006]; NSFC[U0833604]; Chinese Academy of Sciences[KZCX1-YW-14-1]
公开日期	2011-11-09
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/16381]
专题	水生生物研究所_藻类生物学及应用研究中心_期刊论文
通讯作者	Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; lrsong@ihb.ac.cn
推荐引用方式 GB/T 7714	Li, Jie,Ou, Danyun,Zheng, Lingling,et al. Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)[J]. PHYCOLOGICAL RESEARCH,2011,59(3):200-207.
APA	Li, Jie.,Ou, Danyun.,Zheng, Lingling.,Gan, Nanqin.,Song, Lirong.,...&lrsong@ihb.ac.cn.(2011).Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria).PHYCOLOGICAL RESEARCH,59(3),200-207.
MLA	Li, Jie,et al."Applicability of the fluorescein diacetate assay for metabolic activity measurement of Microcystis aeruginosa (Chroococcales, Cyanobacteria)".PHYCOLOGICAL RESEARCH 59.3(2011):200-207.