High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress

doi:10.1111/ppl.12583

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	High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress
	Tanaka, Kiyoshi 3; Wang, Shiwen 1,2; Yin, LN (reprint author), Tottori Univ, Fac Agr, Tottori 6808533, Japan.; Yin, Lina 1,2; Mano, Jun'ichi 4,5; Deng, Xiping 1,2; Zhang, Suiqi 1,2; Zhang, Meijuan 2; Yin, LN (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Yin, LN (reprint author), Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.
刊名	PHYSIOLOGIA PLANTARUM
	2017
卷号	161 期号:2 页码:211-223
ISSN号	0031-9317
DOI	10.1111/ppl.12583
文献子类	Article
英文摘要	Lipid peroxide-derived reactive carbonyl species (RCS), generated downstream of reactive oxygen species (ROS), are critical damage-inducing species in plant aluminum (Al) toxicity. In mammals, RCS are scavenged primarily by glutathione (reduced form of glutathione, GSH), but in plant Al stress, contribution of GSH to RCS detoxification has not been evaluated. In this study, Arabidopsis plants overexpressing the gene AtGR1 (accession code At3g24170), encoding glutathione reductase (GR), were generated, and their performance under Al stress was examined. These transgenic plants (GR-OE plants) showed higher GSH levels and GSH/GSSG (oxidized form of GSH) ratio, and an improved Al tolerance as they suffered less inhibition of root growth than wild-type under Al stress. Exogenous application of 4-hydroxy-2-nonenal, an RCS responsible for Al toxicity in roots, markedly inhibited root growth in wild-type plants. GR-OE plants suffered significantly smaller inhibition, indicating that the enhanced GSH level increased the capacity of RCS detoxification. The generation of H2O2 due to Al stress in GR-OE plants was lower by 26% than in wild-type. Levels of various RCS, such as malondialdehyde, butyraldehyde, phenylacetaldehyde, (E)-2-heptenal and n-octanal, were suppressed by more than 50%. These results indicate that high levels of GSH and GSH/GSSG ratio by GR overexpression contributed to the suppression of not only ROS, but also RCS. Thus, the maintenance of GSH level by overexpressing GR reinforces dual detoxification functions in plants and is an efficient approach to enhance Al tolerance.
学科主题	Plant Sciences
URL标识	查看原文
出版地	HOBOKEN
语种	英语
出版者	WILEY
WOS记录号	WOS:000417402000003
资助机构	National Natural Sciences Foundation of China [31200206]; Northwest AF University; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [A314021403-C5]; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015389]; West Light Foundation of the Chinese Academy of Sciences ; National Natural Sciences Foundation of China [31200206]; Northwest AF University; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [A314021403-C5]; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015389]; West Light Foundation of the Chinese Academy of Sciences ; National Natural Sciences Foundation of China [31200206]; Northwest AF University; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [A314021403-C5]; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015389]; West Light Foundation of the Chinese Academy of Sciences ; National Natural Sciences Foundation of China [31200206]; Northwest AF University; State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [A314021403-C5]; Youth Innovation Promotion Association of the Chinese Academy of Sciences [2015389]; West Light Foundation of the Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.iswc.ac.cn/handle/361005/7903]
专题	水保所科研产出--SCI_2017--SCI
通讯作者	Yin, LN (reprint author), Tottori Univ, Fac Agr, Tottori 6808533, Japan.; Yin, LN (reprint author), Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.; Yin, LN (reprint author), Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China.
作者单位	1.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, Yangling 712100, Peoples R China 2.Northwest A&F Univ, State Key Lab Soil Eros & Dryland Farming Loess P, Inst Soil & Water Conservat, Yangling 712100, Peoples R China 3.Tottori Univ, Fac Agr, Tottori 6808533, Japan 4.Yamaguchi Univ, Sci Res Ctr, Yamaguchi 7538515, Japan 5.Yamaguchi Univ, Grad Sch Agr, Yamaguchi 7538515, Japan
推荐引用方式 GB/T 7714	Tanaka, Kiyoshi,Wang, Shiwen,Yin, LN ,et al. High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress[J]. PHYSIOLOGIA PLANTARUM,2017,161(2):211-223.
APA	Tanaka, Kiyoshi.,Wang, Shiwen.,Yin, LN .,Yin, Lina.,Mano, Jun'ichi.,...&Yin, LN .(2017).High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress.PHYSIOLOGIA PLANTARUM,161(2),211-223.
MLA	Tanaka, Kiyoshi,et al."High level of reduced glutathione contributes to detoxification of lipid peroxide-derived reactive carbonyl species in transgenic Arabidopsis overexpressing glutathione reductase under aluminum stress".PHYSIOLOGIA PLANTARUM 161.2(2017):211-223.