Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application

doi:10.1016/j.procbio.2017.03.026

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application
	Zhao, Fuhua 1,2; Wang, Qiao 1,2; Dong, Jianjun 1; Xian, Mo 2; Yu, Junhong 1; Yin, Hua 1; Chang, Zongming 1; Mu, Xindong 2; Hou, Tonggang 2; Wang, Jianxun 2
刊名	PROCESS BIOCHEMISTRY
	2017-06-01
卷号	57 页码:87-94
关键词	Enzyme-inorganic Nanoflowers Microbeads Alginate Enzyme Immobilization Diacetyl
DOI	10.1016/j.procbio.2017.03.026
文献子类	Article
英文摘要	In this work, novel nanoflower/alginate microbeads were synthesized to immobilize alpha-acetolactate decarboxylase (ALDC) with a facile approach. Typically, ALDC was first coprecipitated with Ca-3(PO4)(2) to form enzyme inorganic hybrid nanoflowers (Ca-3(PO4)(2)-ALDC), and then the nanoflowers containing micrometre-sized particles and nanoscale flower-like petals were entrapped in alginate gel beads (Ca-3(PO4)(2)-ALDC)@Mg). Compared with free ALDC and Ca-3(PO4)(2)-ALDC nanoflowers, the microbeads performed greatly improved recyclability and stability, meanwhile, retained 98% of activity compared with free ALDC. Moreover, the immobilized enzyme can be used in a 300 L beer fermentation tank to prevent the formation of diacetyl, solve the problem of beer off-flavor and short the beer maturation time, thus exhibiting a great potential application in beer brewing industry.
WOS关键词	ALPHA-ACETOLACTATE DECARBOXYLASE ; CA-ALGINATE BEADS ; HYBRID NANOFLOWERS ; GRAPHENE OXIDE ; EFFICIENT METHOD ; ENCAPSULATION ; LIPASE ; MICROCAPSULES ; ENTRAPMENT ; CAPSULES
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种	英语
WOS记录号	WOS:000403121600012
资助机构	National Natural Science Foundation of China(21433001 ; Applied Basic Research Foundation of Qingdao City(14-2-4-21-jch) ; 21603251)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/9650]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	1.Tsingtao Brewery Co Ltd, State Key Lab Biol Fermentat Engn Beer, Qingdao 266100, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Fuhua,Wang, Qiao,Dong, Jianjun,et al. Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application[J]. PROCESS BIOCHEMISTRY,2017,57:87-94.
APA	Zhao, Fuhua.,Wang, Qiao.,Dong, Jianjun.,Xian, Mo.,Yu, Junhong.,...&Wang, Jianxun.(2017).Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application.PROCESS BIOCHEMISTRY,57,87-94.
MLA	Zhao, Fuhua,et al."Enzyme-inorganic nanoflowers/alginate microbeads: An enzyme immobilization system and its potential application".PROCESS BIOCHEMISTRY 57(2017):87-94.