The molecular basis for lipase stereoselectivity

doi:10.1007/s00253-018-8858-z

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 酶工程团队

	The molecular basis for lipase stereoselectivity
	Chen, Hui 1; Meng, Xiao 2; Xu, Xiaoqing 3; Liu, Wenbo 3; Li, Shengying 1
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2018-04-01
卷号	102 期号:8 页码:3487-3495
关键词	Lipase Stereoselectivity Steric Exclusion Structural Flexibility Hydrogen Bond Electrostatic Interaction
ISSN号	0175-7598
DOI	10.1007/s00253-018-8858-z
文献子类	Review
英文摘要	Lipases are among the most applied biocatalysts in organic synthesis to catalyze the kinetic resolution of a wide range of racemic substrates to yield optically pure compounds. Due to the rapidly increased demands for optically pure compounds, deep understanding of the molecular basis for lipase stereoselectivity and how to obtain lipases with excellent asymmetric selectivity have become one of primary research goals in this field. This review is focused on the molecular factors that have impacts on the stereoselectivity of lipases including the steric complementarity between the lipase topological structure and its substrate, the regional structural flexibility, the hydrogen bonds between the residues around the catalytic site and the tetrahedral intermediates, and the electrostatic interactions between surface residues. Moreover, the synergistic effects of these structural factors on the catalytic properties including stereoselectivity, activity, and stability are also discussed.
WOS关键词	CANDIDA-RUGOSA LIPASE ; PSEUDOMONAS-CEPACIA LIPASE ; YARROWIA-LIPOLYTICA LIPASE ; SITE-DIRECTED MUTAGENESIS ; RHIZOMUCOR-MIEHEI LIPASE ; SUBSTRATE-BINDING SITE ; PATH-PLANNING APPROACH ; ORGANIC-SOLVENTS ; CHEMICAL-MODIFICATION ; SECONDARY ALCOHOLS
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000428316700004
资助机构	National Natural Science Foundation of China(NSFC 21406250) ; Applied Basic Research Programs of Science and Technology of Qingdao(15-9-1-106-jch)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10743]
专题	青岛生物能源与过程研究所_酶工程团队
通讯作者	Li, Shengying
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, CAS Key Lab Biofuels, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 2.Shanghai SynTheAll Pharmaceut Co Ltd, 90 Delin Rd Waigaoqiao Pilot Free Trade, Shanghai 200131, Peoples R China 3.Rushan Hanwei Biol Sci & Technol Co Ltd, 306 Qingshan Rd, Rushan 264502, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Chen, Hui,Meng, Xiao,Xu, Xiaoqing,et al. The molecular basis for lipase stereoselectivity[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(8):3487-3495.
APA	Chen, Hui,Meng, Xiao,Xu, Xiaoqing,Liu, Wenbo,&Li, Shengying.(2018).The molecular basis for lipase stereoselectivity.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(8),3487-3495.
MLA	Chen, Hui,et al."The molecular basis for lipase stereoselectivity".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.8(2018):3487-3495.