Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source

doi:10.1080/21655979.2017.1323592

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source
	Xu, Xin 1,2; Xie, Meng 1; Zhao, Qian 3; Xian, Mo 1; Liu, Huizhou 1
刊名	BIOENGINEERED
	2018
卷号	9 期号:1 页码:116-123
关键词	Acetate Assimilation Aeration Mevalonate Two-stage Fed-batch Escherichia Coli
ISSN号	2165-5979
DOI	10.1080/21655979.2017.1323592
文献子类	Article
英文摘要	We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06g L-1 mevalonate with a productivity of 0.03g L-1 h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85g L-1 and 0.13g L-1 h(-1), respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05g L-1 and 4.97g L-1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
WOS关键词	ACETATE ACCUMULATION ; GROWTH ; EXPRESSION ; PROTEIN ; FLUX
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000425053900002
资助机构	National Natural Science Foundation of China(21572242 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; 21376255)
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn/handle/337004/10633]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
通讯作者	Xian, Mo; Liu, Huizhou
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xin,Xie, Meng,Zhao, Qian,et al. Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source[J]. BIOENGINEERED,2018,9(1):116-123.
APA	Xu, Xin,Xie, Meng,Zhao, Qian,Xian, Mo,&Liu, Huizhou.(2018).Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.BIOENGINEERED,9(1),116-123.
MLA	Xu, Xin,et al."Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source".BIOENGINEERED 9.1(2018):116-123.