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Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP
He, Linwen1,2; Zhu, Jianyi3; Lu, Qinqin4; Niu, Jianfeng1; Zhang, Baoyu1; Lin, Apeng1; Wang, Guangce1
刊名JOURNAL OF PHYCOLOGY
2013-06-01
卷号49期号:3页码:517-522
关键词AFLP archeospore conchospore genetic similarity Pyropia yezoensis
ISSN号0022-3646
DOI10.1111/jpy.12058
英文摘要Pyropia yezoensis (Ueda) M. S. Hwang et H. G. Choi (previously called Porphyra yezoensis) is an economically important alga. The blades generated from conchospores are genetic chimeras, which are not suitable for genetic similarity analysis. In this study, two types of blades from a single filament of P.yezoensis sporophyte filament were obtained. One type, ConB, consisted of 40 blades that had germinated from conchospores. The other type, ArcB, consisted of 88 blades that had germinated from archeospores released from ConB. Both of them were analyzed by amplified fragment length polymorphism. The low genetic similarity levels for both conchospore-germinated and archeospore-germinated blades demonstrated that the conchcelis we used was cross-fertilized. Furthermore, a higher polymorphic loci ratio (98.6%) was detected in ArcB than in ConB (80.7%), and the average genetic similarity of ArcB (average 0.61) was lower than that of ConB (average 0.71). These differences indicated that genetic analysis using ArcB gives more accurate results.
资助项目863 project[2012AA100806] ; 973 project[2011CB411908] ; National Natural Science Foundation of China[40906074] ; nonprofit research project for the State Oceanic Administration[201105023-2] ; nonprofit research project for the State Oceanic Administration[201105023-7] ; Ministry of Agriculture of China[200903030]
WOS研究方向Plant Sciences ; Marine & Freshwater Biology
语种英语
出版者WILEY-BLACKWELL
WOS记录号WOS:000319874900009
内容类型期刊论文
源URL[http://ir.qdio.ac.cn/handle/337002/154608]  
专题中国科学院海洋研究所
通讯作者Wang, Guangce
作者单位1.Chinese Acad Sci IOCAS, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Changshu Inst Technol, Dept Biol & Food Engn, Changshu 215500, Peoples R China
4.Jiangsu Inst Oceanol & Marine Fisheries, State Germplasm Bank Porphyra, Nantong 226007, Peoples R China
推荐引用方式
GB/T 7714
He, Linwen,Zhu, Jianyi,Lu, Qinqin,et al. Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP[J]. JOURNAL OF PHYCOLOGY,2013,49(3):517-522.
APA He, Linwen.,Zhu, Jianyi.,Lu, Qinqin.,Niu, Jianfeng.,Zhang, Baoyu.,...&Wang, Guangce.(2013).Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP.JOURNAL OF PHYCOLOGY,49(3),517-522.
MLA He, Linwen,et al."Genetic similarity analysis within Pyropia yezoensis blades developed from both conchospores and blade archeospores using AFLP".JOURNAL OF PHYCOLOGY 49.3(2013):517-522.
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